Effects of DL‐alpha‐tocopheryl succinate in combination with sodium butyrate and camp stimulating agent on neuroblastoma cells in culture

The effect of dl‐alpha‐tocopheryl (vitamin E) succinate in combination with Prostaglandin A2 (PGA2) and sodium butyrate on mouse neuroblastoma cells (NBP2) in culture, according to the criteria of growth inhibition and morphological differentiation (neurite formation), was studied. Results showed that PGA2 and sodium butyrate inhibited the growth of NB cells in a dose‐dependent manner. The combined effects of vitamin E succinate with PGA2 or sodium butyrate, according to the criterion of growth inhibition, were additive. Vitamin E succinate by itself did not induce morphological differentiation, but it enhanced PGA2‐induced morphological differentiation. Sodium butyrate alone or in combination with vitamin E succinate did not significantly increase the level of morphological differentiation. Sodium succinate and an equal amount of solvent (ethanol) failed to modify the effect of PGA2 or sodium butyrate. This suggests that the effect of vitamin E succinate in modifying the response of PGA2 and sodium butyrate on NB cells is due to the effect of vitamin E rather than to that of succinate.

[1]  K. Prasad,et al.  dl-α-Tocopheryl Succinate Enhances the Effect of γ-lrradiation on Neuroblastoma Cells in Culture 1 2 , 1984, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[2]  K. Prasad,et al.  Study on the Specificity of α-Tocopheryl (Vitamin E) Acid Succinate Effects on Melanoma, Glioma and Neuroblastoma Cells in Culture 1 2 , 1983 .

[3]  A. Ravid,et al.  Effect of polar organic compounds on leukemic cells: Butyrate‐induced partial remission of acute myelogenous leukemia in a child , 1983, Cancer.

[4]  K. Prasad,et al.  Effects of tocopherol (vitamin E) acid succinate on morphological alterations and growth inhibition in melanoma cells in culture. , 1982, Cancer research.

[5]  K. Prasad,et al.  Butyric acid: a small fatty acid with diverse biological functions. , 1980, Life sciences.

[6]  K. Prasad,et al.  Vitamin E Increases the Growth Inhibitory and Differentiating Effects of Tumor Therapeutic Agents on Neuroblastoma and Glioma Cells in Culture 1 , 1980, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[7]  K. Prasad,et al.  Vitamin E Induces Morphological Differentiation and Increases the Effect of Ionizing Radiation on Neuroblastoma Cells in Culture 1 , 1979, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[8]  K. Prasad,et al.  Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture , 1977, Nature.

[9]  S. Das,et al.  A rationale for the treatment of metastatic neuroblastoma. , 1976, Journal of the National Cancer Institute.

[10]  K. Prasad.  DIFFERENTIATION OF NEUROBLASTOMA CELLS IN CULTURE , 1975, Biological reviews of the Cambridge Philosophical Society.

[11]  J. Lucy,et al.  The biochemical modes of action of vitamin e and selenium: A hypothesis , 1973, FEBS letters.

[12]  K. Prasad,et al.  Basal level of neurotransmitter synthesizing enzymes and effect of cyclic AMP agents on the morphological differentiation of isolated neuroblastoma clones. , 1973, Nature: New biology.

[13]  A. Tappel,et al.  VITAMIN E AND FREE RADICAL PEROXIDATION OF LIPIDS * , 1972, Annals of the New York Academy of Sciences.

[14]  K. Prasad,et al.  Effect of DL-glyceraldehyde on mouse neuroblastoma cells in culture. , 1972, Cancer research.

[15]  P. J. Rhodes,et al.  The Athenian boule , 1973 .