In vitro evaluation of cell/biomaterial interaction by MTT assay.

The tetrazolium-based colorimetric assay (MTT test) measures only in vitro living cells and the results are directly related to the number of viable cultured cells. It has been adopted in immunological investigations, cancer research and, recently, biocompatibility evaluation. We used the MTT method with minor modifications to fit it to an in vitro study of biomaterial-cell interactions. The MTT assay was confirmed to be feasible, rapid and reproducible. Moreover, it showed a good correlation with other in vitro proliferation assays, such as the 3H-thymidine uptake assay. By using the MTT method and the ASTM procedure for extracting biomaterials, we quantified the in vitro cell compatibility of different metals and polymers.

[1]  D. P. Bentley,et al.  In vitro chemosensitivity testing in chronic lymphocytic leukaemia patients. , 1991, Leukemia research.

[2]  C. Taylor,et al.  A feasibility study of the MTT assay for chemosensitivity testing in ovarian malignancy. , 1990, British Journal of Cancer.

[3]  D. Sgouras,et al.  Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1 — Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation ofin vitro cytotoxicity , 1990 .

[4]  D. Ducassou,et al.  In vitro evaluation of an epoxy resin's cytocompatibility using cell lines and human differentiated cells. , 1991, Journal of biomaterials science. Polymer edition.

[5]  J. G. Cory,et al.  Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. , 1991, Cancer communications.

[6]  M. Harmand,et al.  Study of the effect of the surface state on the cytocompatibility of a Co-Cr alloy using human osteoblasts and fibroblasts. , 1990, Journal of biomedical materials research.

[7]  Stanley A. Brown,et al.  In vitro biocompatibility of polyetheretherketone and polysulfone composites. , 1990, Journal of biomedical materials research.

[8]  U. Schauer,et al.  Detection of activated lymphocyte subsets by fluorescence and MTT staining. , 1989, Journal of immunological methods.

[9]  R G Craig,et al.  The in vitro effects of metal cations on eukaryotic cell metabolism. , 1991, Journal of biomedical materials research.

[10]  T. Hanawa,et al.  Cytotoxicities of oxides, phosphates and sulphides of metals. , 1992, Biomaterials.

[11]  M. Sefton,et al.  Colorimetric assay for cellular activity in microcapsules. , 1990, Biomaterials.

[12]  S. Das,et al.  Assessment of the lymphocyte response to silicone. , 1990, Plastic and reconstructive surgery.

[13]  D. Gerlier,et al.  Use of MTT colorimetric assay to measure cell activation. , 1986, Journal of immunological methods.

[14]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[15]  M. Rosdy,et al.  Proliferation of normal human keratinocytes on silicone substrates. , 1991, Biomaterials.

[16]  A. Isetta,et al.  MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. , 1990, Journal of immunological methods.

[17]  M K Patterson,et al.  Measurement of growth and viability of cells in culture. , 1979, Methods in enzymology.

[18]  M. Koyama,et al.  An improved colorimetric assay for interleukin 2. , 1986, Journal of immunological methods.

[19]  F. Denizot,et al.  Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. , 1986, Journal of immunological methods.

[20]  G. Ossenkoppele,et al.  Cell mediated cytotoxicity against U 937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study. , 1991, Journal of immunological methods.

[21]  T. Mosmann,et al.  Specific assays for cytokine production by T cells. , 1989, Journal of immunological methods.

[22]  S. Kitada,et al.  Proliferation of human ocular fibroblasts. An assessment of in vitro colorimetric assays. , 1990, Investigative ophthalmology & visual science.

[23]  M. Hansen,et al.  Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. , 1989, Journal of immunological methods.

[24]  C. Ware,et al.  Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. , 1984, Journal of immunological methods.

[25]  G. Caughman,et al.  Correlation of cytotoxicity, filler loading and curing time of dental composites. , 1991, Biomaterials.