FIRST DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF FLUOXETINE HYDROCHLORIDE AND OLANZAPINE IN TABLETS

Fluoxetine hydrochloride (FH) in combination with Olanzapine (OZ) is used in treatment of depressive episodes associated with bipolar disorder. The first derivative spectrophotometric method for simultaneous determination of fluoxetine hydrochloride (FH) and olanzapine (OZ) from two component tablet dosage form has been developed and validated. Chromatographic methods viz. HPLC and HPTLC methods were reported in the literature for the simultaneous determination of FH and OZ from combined dosage forms. In the present investigation an attempt has been made to develop accurate, reproducible, rapid and cost-effective method for simultaneous determination of FH and OZ in pharmaceutical formulation. The wavelengths selected for determination of FH and OZ is 235.5nm and 296nm, respectively. The beer’s law was obeyed in the concentration range of 8-80μg/mL and 220μg/mL for Fluoxetine and Olanzapine, respectively. The proposed methods were validated and successfully applied to the determination of FH and OZ in tablet formulations. The results suggested that the proposed procedures can be used for routine quality control of tablets containing FH and OZ. INTRODUCTION: Fluoxetine hydrochloride (FH) in combination with Olanzapine (OZ) is used in treatment of depressive episodes associated with bipolar disorder . This combination of FH and OZ produce robust and sustained increases of extracellular levels of dopamine and norepinephrine, which were significantly greater than with either drug alone . This combination drugs are available in tablet dosage form in the market. The combination of FH and OZ is not official in any pharmacopoeia. The FH, a selective serotonin reuptake inhibitor, is chemically d, lNmethyl3phenyl-3-*α, α, αtrifluoroptolyl) oxy] propyl amine hydrochloride (Figure 1a). Chemically, olanzapine is 2-methyl-4-(4-methyl-1piperazinyl)-10H [2, 3-b] [1, 5] benzodiazepine (Figure 1b). FIG. 1A: FLUOXETINE HYDROCHLORIDE