Effect of vitamin E supplementation on superoxide and malondialdehyde generation in acute celphos poisoning

Introduction: Aluminum phosphide is one of the most commonly used grain fumigants and aluminum phosphide poisoning (ALP) has been reported as the most common cause of acute poisoning in India. Aluminum toxicity has been reported to increase the rate of lipid peroxidation and free radical formation. Materials and Methods: The present study was designed to investigate the role of vitamin E supplementation on free radical generation and lipid peroxidation in acute aluminium phosphide poisoning in rats. Thirty disease free albino rats were taken to study the effect of acute aluminium phosphide poisoning (ALP poisoning) were further divided into 3 subgroups of ten rats each: A, B and C. Group A: given vehicle (Ginni Oil) only. Group B: given 5 ml 'celphos mixture' (or 0.3mg/g body wt.). Group C: rats with acute Celphos poisoning along with vitamin E (1.5 mg vitamin E/g body weight of rat. The MDA levels and superoxide levels (Nitroblue tetrazolium (NBT) reduction) were estimated. Results: MDA levels were significantly higher in the group B as compared to Group A. In group C, administration of vitamin E resulted in decreased MDA level compared to group B. MDA levels in group C still remained significantly higher as compared to group A. NBT reduction was significantly increased in group B as compared to group A. Administration of vitamin E to rats of group C resulted in significant decrease of NBT reduction. Conclusion: Findings of the present study showed that vitamin E via its antioxidant action and anti-inflammatory effects has protective effect on phosphine-induced toxicity in rats.

[1]  N. Soliman,et al.  Aluminium Phosphide-induced Hepato-nephrotoxicity and OxidativeDamage in Rats: The Protective Effect of α-lipoic Acid , 2015 .

[2]  A. Nosrati,et al.  Aluminum Phosphide Poisoning: A Case Series in North Iran , 2013 .

[3]  A. Moghadamnia.  An update on toxicology of aluminum phosphide , 2012, DARU Journal of Pharmaceutical Sciences.

[4]  H. Mohammadi,et al.  Aluminium Phosphide Poisoning and Oxidative Stress , 2012, Journal of Medical Toxicology.

[5]  M. Abdollahi,et al.  A retrospective 7-years study of aluminum phosphide poisoning in Tehran , 2010 .

[6]  A. Mathai,et al.  Acute aluminium phosphide poisoning: Can we predict mortality? , 2010, Indian journal of anaesthesia.

[7]  A. Agrawal,et al.  The Successful Treatment Of Aluminium Phosphide Poisoning With Limited Resources , 2010 .

[8]  P. Ebert,et al.  Mitochondrial uncouplers act synergistically with the fumigant phosphine to disrupt mitochondrial membrane potential and cause cell death. , 2008, Toxicology.

[9]  S. Wahab,et al.  Acute Aluminium Phosphide Poisoning: An Update , 2008 .

[10]  M. L. Alvarez,et al.  Involvement of oxidative stress in the impairment in biliary secretory function induced by intraperitoneal administration of aluminum to rats , 2007, Biological Trace Element Research.

[11]  Pallavi Sethi,et al.  Bacopa monniera prevents from aluminium neurotoxicity in the cerebral cortex of rat brain. , 2007, Journal of ethnopharmacology.

[12]  J. Winther,et al.  Erv2p: characterization of the redox behavior of a yeast sulfhydryl oxidase. , 2007, Biochemistry.

[13]  G. Turgut,et al.  Changes in the levels of MDA and GSH in mice , 2006 .

[14]  A. Bindoli,et al.  Induction of mitochondrial permeability transition by auranofin, a Gold(I)‐phosphine derivative , 2002, British journal of pharmacology.

[15]  J. Féher,et al.  Vitamin E protects against iron-hexachlorobenzene induced porphyria and formation of 8-hydroxydeoxyguanosine in the liver of C57BL/10ScSn mice. , 2001, Toxicology letters.

[16]  C. Chow.  Vitamin E Regulation of Mitochondrial Superoxide Generation , 2001, Neurosignals.

[17]  S. Gupta,et al.  Aluminium Phosphide Poisoning: A Challenge for the Physician , 2001 .

[18]  J. Casida,et al.  Phosphine-induced oxidative damage in rats: attenuation by melatonin. , 2000, Free radical biology & medicine.

[19]  H. Nagahata,et al.  Effects of vitamins A and E on superoxide production and intracellular signaling of neutrophils in Holstein calves. , 2000, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[20]  K. Chugh,et al.  A critical evaluation of anti-peroxidant effect of intravenous magnesium in acute aluminium phosphide poisoning. , 1997, Magnesium Research.

[21]  D. Nathan,et al.  Quantitative nitroblue tetrazolium test in chronic granulomatous disease. , 1968, The New England journal of medicine.

[22]  B. Johnson,et al.  Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. , 1966, Analytical biochemistry.