Monoclonal antibody that recognizes a domain on heterogeneous nuclear ribonucleoprotein K and PTB-associated splicing factor.

hnRNP K protein is a member of the heterogeneous nuclear protein (hnRNP) complex that, besides its function as a translational regulator of human mRNA, is also considered to be a transcription factor involved in tumorigenesis. PSF is a protein part of the human spliceosome and essential in RNA splicing. Here we report the generation of one monoclonal antibody GG6H9.1C3 that recognized both hnRNP K and PSF proteins using Western blot analysis, flow cytometry, and immunocytochemistry.

[1]  Douglas S. Portman,et al.  The hnRNP proteins , 1993, Molecular Biology Reports.

[2]  M. Swanson,et al.  hnRNP complexes: composition, structure, and function. , 1999, Current opinion in cell biology.

[3]  E. Kremmer,et al.  mRNA Silencing in Human Erythroid Cell Maturation , 2008, Journal of Biological Chemistry.

[4]  D. Solter,et al.  Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells , 1982, Cell.

[5]  D. Helfman,et al.  The use of antibodies to the polypyrimidine tract binding protein (PTB) to analyze the protein components that assemble on alternatively spliced pre-mRNAs that use distant branch points. , 1998, RNA.

[6]  Y. Shav-Tal,et al.  A hematopoietic organ-specific 49-kD nuclear antigen: predominance in immature normal and tumor granulocytes and detection in hematopoietic precursor cells. , 1996, Blood.

[7]  G. Dreyfuss,et al.  Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein , 1992, Molecular and cellular biology.

[8]  E. Viñuela,et al.  An erythroid species-specific antigen of swine detected by a monoclonal antibody. , 1992, Hybridoma.

[9]  Jerzy Ostrowski,et al.  hnRNP K: One protein multiple processes , 2004, BioEssays : news and reviews in molecular, cellular and developmental biology.

[10]  P. Avner,et al.  Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. , 1984, Hybridoma.

[11]  L. Shkreta,et al.  hnRNP proteins and splicing control. , 2007, Advances in experimental medicine and biology.

[12]  S. Vicent,et al.  Altered patterns of expression of members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family in lung cancer. , 2003, Lung cancer.

[13]  Y. Shav-Tal,et al.  PSF and p54nrb/NonO – multi‐functional nuclear proteins , 2002, FEBS letters.

[14]  M. Mandal,et al.  Growth Factors Regulate Heterogeneous Nuclear Ribonucleoprotein K Expression and Function* , 2001, The Journal of Biological Chemistry.

[15]  C. Arce,et al.  Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes. , 2002, Veterinary immunology and immunopathology.

[16]  G I Murray,et al.  Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer , 2006, British Journal of Cancer.

[17]  David Levens,et al.  Heterogeneous nuclear ribonucleoprotein K is a transcription factor , 1996, Molecular and cellular biology.

[18]  J. G. Patton,et al.  Cloning and characterization of PSF, a novel pre-mRNA splicing factor. , 1993, Genes & development.

[19]  Björn Rozell,et al.  A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. , 2003, Human reproduction.

[20]  D. Solter,et al.  Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Moore.  From Birth to Death: The Complex Lives of Eukaryotic mRNAs , 2005, Science.