Immunosensors: Principles and Applications

Abstract This paper presents an overview of the principles and applications of immunofluorescence spectroscopy in the development of antibody-based fiber-optic sensors. Special focus is devoted to antibody-based fiber-optic fluoroimmunosensors developed in our laboratories to detect important pollutants and biochemicals. The fiber-optic sensor utilizes antibodies covalently bound or encapsulated on the probe tip. The usefulness of fluoroimmunosensors for environmental and biomedical monitoring is discussed.