Amperometric Tyrosinase Biosensor Based on Carbon Nanotube-Doped Sol-Gel-Derived Zinc Oxide–Nafion Composite Films

A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)–ZnO–Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17 nm) of the composite film, the biosensor exhibits very fast response time of 2 s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7×10−8 M (S/N=3) for phenol. The biosensor retains 88 % of its initial activity after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.

[1]  J. Song,et al.  Production of Hydrogen-Capped Polyynes by Laser Ablation of Graphite in Neat Water , 2009 .

[2]  Han Nim Choi,et al.  Amperometric Tyrosinase Biosensor Based on Carbon Nanotube–Titania–Nafion Composite Film , 2007 .

[3]  Han Nim Choi,et al.  Tris(2,2′‐bipyridyl)ruthenium(II) Electrogenerated Chemiluminescence Sensor Based on Sol–Gel‐Derived V2O5/Nafion Composite Films , 2006 .

[4]  Guo-Li Shen,et al.  A Mediator-Free Tyrosinase Biosensor Based on ZnO Sol-Gel Matrix , 2005 .

[5]  Guo-Li Shen,et al.  A phenol biosensor based on immobilizing tyrosinase to modified core-shell magnetic nanoparticles supported at a carbon paste electrode , 2005 .

[6]  Zhennan Gu,et al.  Determination of Phenolic Compounds Based on the Tyrosinase‐ Single Walled Carbon Nanotubes Sensor , 2005 .

[7]  Huangxian Ju,et al.  Mediator-free phenol sensor based on titania sol-gel encapsulation matrix for immobilization of tyrosinase by a vapor deposition method. , 2003, Biosensors & bioelectronics.

[8]  J. Kong,et al.  A sensitive mediator-free tyrosinase biosensor based on an inorganic-organic hybrid titania sol-gel matrix , 2003 .

[9]  Han Nim Choi,et al.  Electrogenerated chemiluminescence from tris(2,2'-bipyridyl)ruthenium(II) immobilized in titania-perfluorosulfonated ionomer composite films. , 2003, Analytical chemistry.

[10]  Won-Yong Lee,et al.  Amperometric phenol biosensor based on sol–gel silicate/Nafion composite film , 2003 .

[11]  S. Dong,et al.  Amperometric tyrosinase biosensor based on a sol–gel-derived titanium oxide–copolymer composite matrix for detection of phenolic compounds , 2001 .

[12]  J. Kong,et al.  Probing trace phenols based on mediator-free alumina sol--gel-derived tyrosinase biosensor. , 2000, Analytical chemistry.

[13]  S. Dong,et al.  Silica sol-gel composite film as an encapsulation matrix for the construction of an amperometric tyrosinase-based biosensor. , 2000, Biosensors & bioelectronics.

[14]  Juan Li,et al.  Silica sol–gel immobilized amperometric biosensor for the determination of phenolic compounds , 1998 .

[15]  Chris W. Brown,et al.  Sol-gel glass as a matrix for chemical and biochemical sensing , 1997 .

[16]  L. Gorton,et al.  Improvement of Electrochemical Biosensors Using Enzyme Immobilization from Water−Organic Mixtures with a High Content of Organic Solvent , 1996 .

[17]  D. Barceló,et al.  Determination of phenolic compounds in water and waste water , 1996 .

[18]  Joseph Wang,et al.  Remote electrochemical biosensor for field monitoring of phenolic compounds , 1995 .

[19]  Joseph Wang,et al.  Microfabricated phenol biosensors based on screen printing of tyrosinase containing carbon ink , 1995 .

[20]  Klaus-Dieter Vorlop,et al.  Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols , 1995 .

[21]  Bruce Dunn,et al.  Sol-gel encapsulation methods for biosensors , 1994 .

[22]  D. Barceló,et al.  Surface water analysis (trace-organic contaminants) and EC regulations , 1994 .

[23]  C. Tillyer,et al.  The development of a catechol enzyme electrode and its possible use for the diagnosis and monitoring of neural crest tumours. , 1991, Biosensors & bioelectronics.

[24]  N. Horowitz,et al.  [77] Tyrosinase (Neurospora crassa)☆☆☆ , 1970 .