PCR-RFLP WITH ALU I RESTRICTION ENZYME IN TLR4 GENE OF SAHIWAL CATTLE

Toll Like Receptor 4 gene (TLR4) is a cell-surface receptor that recognizes a broad class of pathogenassociated molecular patterns, activates innate and adaptive immune responses, and plays an important role in pathogen defense. In 190 Sahiwal cattle PCR-RFLP technique was carried out with the help of Alu I restriction enzyme. In 190 Sahiwal cattle PCR-RFLP technique was carried out with the help of Alu I restriction enzyme. Monomorphic patterns were detected in the primers 3.1, 3.5 and 3.6 of TLR4 gene in 190 Sahiwal cattle.