Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8).

The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.

[1]  P. A. Harding,et al.  Purification and Characterization of Novel Heparin-binding Growth Factors in Uterine Secretory Fluids , 1997, The Journal of Biological Chemistry.

[2]  T. Sugimoto,et al.  Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA. , 1997, Biochemical and biophysical research communications.

[3]  I. Lax,et al.  Regulation of growth factor activation by proteoglycans: What is the role of the low affinity receptors? , 1995, Cell.

[4]  D. Moscatelli,et al.  Fibroblast Growth Factor-2 Can Mediate Cell Attachment by Linking Receptors and Heparan Sulfate Proteoglycans on Neighboring Cells (*) , 1995, The Journal of Biological Chemistry.

[5]  Gary R. Grotendorst,et al.  Significant correlation between connective tissue growth factor gene expression and skin sclerosis in tissue sections from patients with systemic sclerosis. , 1995, The Journal of investigative dermatology.

[6]  H. Ihn,et al.  Growth regulation in scleroderma fibroblasts: increased response to transforming growth factor-beta 1. , 1995, The Journal of investigative dermatology.

[7]  M. Lyon,et al.  Interaction of hepatocyte growth factor with heparan sulfate. Elucidation of the major heparan sulfate structural determinants. , 1994, The Journal of biological chemistry.

[8]  M. Takigawa,et al.  Proto‐oncogene Expression in a Human Chondrosarcoma Cell Line: HCS‐2/8 , 1994, Japanese journal of cancer research : Gann.

[9]  S. Ayad,et al.  Conformation dependence of integrin-type II collagen binding. Inability of collagen peptides to support alpha 2 beta 1 binding, and mediation of adhesion to denatured collagen by a novel alpha 5 beta 1-fibronectin bridge. , 1994, Journal of cell science.

[10]  P. Bork.  The modular architecture of a new family of growth regulators related to connective tissue growth factor , 1993, FEBS letters.

[11]  Gary R. Grotendorst,et al.  Regulation of connective tissue growth factor gene expression in human skin fibroblasts and during wound repair. , 1993, Molecular biology of the cell.

[12]  Jeffrey L. Wrana,et al.  TGFβ signals through a heteromeric protein kinase receptor complex , 1992, Cell.

[13]  M. Adolphe.  Biological Regulation of the Chondrocytes , 1992 .

[14]  M. Takigawa,et al.  Demonstration of receptors for epidermal growth factor on cultured rabbit chondrocytes and regulation of their expression by various growth and differentiation factors. , 1992, Biochemical and biophysical research communications.

[15]  Gary R. Grotendorst,et al.  Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10 , 1991, The Journal of cell biology.

[16]  M. Takigawa,et al.  Effects of various growth and differentiation factors on expression of parathyroid hormone receptors on rabbit costal chondrocytes in culture. , 1991, Endocrinology.

[17]  M. Takigawa,et al.  Establishment from a human chondrosarcoma of a new immortal cell line with high tumorigenicity in vivo, which is able to form proteoglycan‐rich cartilage‐like nodules and to respond to insulin in vitro , 1991, International journal of cancer.

[18]  Y. Takano,et al.  Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes. , 1989, Cancer research.

[19]  R. Ross,et al.  Platelet-derived growth factor. III. Identification of a platelet-derived growth factor receptor by affinity labeling. , 1982, The Journal of biological chemistry.

[20]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[21]  Gary R. Grotendorst,et al.  Transforming growth factor beta induces anchorage-independent growth of NRK fibroblasts via a connective tissue growth factor-dependent signaling pathway. , 1997, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.