Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli.

The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice.

[1]  G. Strichartz,et al.  Purification and characterization of chlorotoxin, a chloride channel ligand from the venom of the scorpion. , 1993, The American journal of physiology.

[2]  M. Tomalski,et al.  Synthesis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using baculovirus vectors. , 1988, Gene.

[3]  C. Chi,et al.  Expression and purification of the BmK M1 neurotoxin from the scorpion Buthus martensii Karsch. , 1999, Protein expression and purification.

[4]  C. Chi,et al.  The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch. , 1999, Toxicon : official journal of the International Society on Toxinology.

[5]  Seymour Ehrenpreis,et al.  Methods in narcotics research , 1975 .

[6]  S. Terakawa,et al.  Purification and the partial amino acid sequence of an insect neurotoxin from the venom of scorpion Buthus martensi Karsch. , 1988, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.

[7]  Xiaoping Zhou,et al.  Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch. , 1989, The Biochemical journal.

[8]  L. Possani,et al.  Peptide toxins as probes of ryanodine receptor structure and function. , 1998, Trends in cardiovascular medicine.

[9]  W. Liu,et al.  Molecular characterization of an anti-epilepsy peptide from the scorpion Buthus martensi Karsch. , 2001, European journal of biochemistry.

[10]  R. Guan,et al.  A new insect neurotoxin AngP1 with analgesic effect from the scorpion Buthus martensii Karsch: purification and characterization. , 2001, The journal of peptide research : official journal of the American Peptide Society.

[11]  C. Chi,et al.  Molecular characterization of a new excitatory insect neurotoxin with an analgesic effect on mice from the scorpion Buthus martensi Karsch. , 1999, Toxicon : official journal of the International Society on Toxinology.

[12]  J. O'Callaghan,et al.  Quantification of the analgesic activity of narcotic antagonists by a modified hot-plate procedure. , 1975, The Journal of pharmacology and experimental therapeutics.

[13]  F. Miranda,et al.  Sur les neurotoxines de deux especes de scorpions nord-africains , 1964 .

[14]  R. Guan,et al.  A depressant insect toxin with a novel analgesic effect from scorpion Buthus martensii Karsch. , 2001, Biochimica et biophysica acta.

[15]  J. Bloomquist,et al.  Expression of a gene encoding a scorpion insectotoxin peptide in yeast, bacteria and plants. , 1992, Gene.

[16]  R. Guan,et al.  Crystallization and preliminary X-ray analysis of a depressant insect toxin from the scorpion Buthus martensii Karsch. , 2001, Acta crystallographica. Section D, Biological crystallography.

[17]  R. Guan,et al.  Crystallization and preliminary X-ray analyses of insect neurotoxins with analgesic effect from the scorpion Buthus martensii Karsch. , 2000, Acta crystallographica. Section D, Biological crystallography.

[18]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[19]  I. Huys,et al.  Electrophysiological characterization of BmK M1, an α‐like toxin from Buthus martensi Karsch venom , 2001, FEBS Letters.

[20]  C. Chi,et al.  The CDNA and genomic DNA sequences of a mammalian neurotoxin from the scorpion Buthus martensii Karsch. , 1997, Toxicon : official journal of the International Society on Toxinology.

[21]  Z. Zeng,et al.  Crystal structure of an acidic neurotoxin from scorpion Buthus martensii Karsch at 1.85 A resolution. , 1996, Journal of molecular biology.

[22]  H. Schägger,et al.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. , 1987, Analytical biochemistry.

[23]  R. Latorre,et al.  Charybdotoxin, a protein inhibitor of single Ca2+-activated K+ channels from mammalian skeletal muscle , 1985, Nature.

[24]  W. Catterall.  Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. , 1980, Annual review of pharmacology and toxicology.

[25]  A. Maelicke,et al.  Selective blockage of voltage-dependent K+ channels by a novel scorpion toxin , 1982, Nature.