Human liver protein map: Update 1993

This publication updates the reference human liver protein map. By microsequencing, 27 spots or 34 polypeptide chains were identified. The most abundant polypeptides detected on the silver stained liver map were key elements in major hepatic biochemical pathways. The new polypeptides and previously known proteins are listed in a table and/or labeled on the protein map, thus providing the 1993 reference human liver SWISS‐2DPAGE database. SWISS‐2DPAGE and the SWISS‐PROT protein sequence databases are closely linked together through the use of common accession numbers.

[1]  Joël Vandekerckhove,et al.  Comprehensive two‐dimensional gel protein databases offer a global approach to the analysis of human cells: The transformed amnion cells (AMA) master database and its link to genome DNA sequence data , 1990, Electrophoresis.

[2]  F. Lottspeich,et al.  Combination of two‐dimensional gel electrophoresis with microsequencing and amino acid composition analysis: Improvement of speed and sensitivity in protein characterization , 1990, Electrophoresis.

[3]  A. Görg,et al.  Two‐dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare): Method, reproducibility and genetic aspects , 1988, Electrophoresis.

[4]  R. Lüthy,et al.  Disulfide bond assignment in human J chain and its covalent pairing with immunoglobulin M. , 1992, Biochemistry.

[5]  D F Hochstrasser,et al.  Methods for increasing the resolution of two-dimensional protein electrophoresis. , 1988, Analytical biochemistry.

[6]  C. Merril,et al.  Simplified silver protein detection and image enhancement methods in polyacrylamide gels , 1982 .

[7]  M. Ackenheil,et al.  Analysis of cerebrospinal fluid from patients with psychiatric and neurological disorders by two‐dimensional electrophoresis: Identification of disease‐associated polypeptides as fibrin fragments , 1991, Electrophoresis.

[8]  C R Merril,et al.  Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[9]  Amos Bairoch,et al.  Plasma and red blood cell protein maps: Update 1993 , 1993, Electrophoresis.

[10]  B. Oakley,et al.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. , 1980, Analytical biochemistry.

[11]  S. Fey,et al.  Expression of the transformation-sensitive protein "cyclin" in normal human epidermal basal cells and simian virus 40-transformed keratinocytes. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[12]  J R Scherrer,et al.  The MELANIE project: From a biopsy to automatic protein map interpretation by computer , 1991, Electrophoresis.

[13]  A. Görg,et al.  Horizontal two‐dimensional electrophoresis with immobilized pH gradients using PhastSystem , 1988, Electrophoresis.

[14]  J. Celis,et al.  Major proteins in normal human lymphocyte subpopulations separated by fluorescence-activated cell sorting and analyzed by two-dimensional gel electrophoresis. , 1988, Leukemia.

[15]  J. Celis,et al.  Secreted proteins from normal and SV40 transformed human MRC-5 fibroblasts: toward establishing a database of human secreted proteins. , 1987, Leukemia.

[16]  T. Rabilloud A comparison between low background silver diammine and silver nitrate protein stains , 1992, Electrophoresis.

[17]  P. Matsudaira,et al.  Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. , 1987, The Journal of biological chemistry.

[18]  Joël Vandekerckhove,et al.  Two‐dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes , 1990, Electrophoresis.

[19]  R. Kuick,et al.  Approach to stationary two‐dimensional pattern: Influence of focusing time and Immobiline/carrier ampholytes concentrations , 1988, Electrophoresis.

[20]  Angelika Görg,et al.  Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients , 1988 .

[21]  R D Appel,et al.  SWISS‐2DPAGE: A database of two‐dimensional gel electrophoresis images , 1993, Electrophoresis.

[22]  C Pasquali,et al.  Plasma protein map: An update by microsequencing , 1992, Electrophoresis.

[23]  D. Hochstrasser,et al.  The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences , 1993, Electrophoresis.

[24]  F. Lottspeich,et al.  Blotting efficiency investigated by using two‐dimensional electrophoresis, hydrophobic membranes and proteins from different sources , 1990, Electrophoresis.

[25]  V. McKusick Mendelian Inheritance in Man: Catalogs of Autosomal Dominant, Autosomal Recessive, and X-Linked Phenotypes , 1983 .

[26]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[27]  H. Leffers,et al.  Computerized, comprehensive databases of cellular and secreted proteins from normal human embryonic lung MRC‐5 fibroblasts: Identification of transformation and/or proliferation sensitive proteins , 1989, Electrophoresis.

[28]  Guy Bauw,et al.  A two‐dimensional gel protein database of noncultured total normal human epidermal keratinocytes: Identification of proteins strongly up‐regulated in psoriatic epidermis , 1990, Electrophoresis.

[29]  F. Lottspeich,et al.  Identification of mouse brain proteins after two‐dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis , 1988, Electrophoresis.

[30]  J. Celis,et al.  Identification of two human phosphoproteins (dividin and IEF 59dl) that are first detected late in G1 near the G1/S transition border of the cell cycle. , 1987, Leukemia.

[31]  Amos Bairoch,et al.  The SWISS-PROT protein sequence data bank, recent developments , 1993, Nucleic Acids Res..

[32]  H. Leffers,et al.  The MRC‐5 human embryonal lung fibroblast two‐dimensional gel cellular protein database: Quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV 40 transformed cells , 1990, Electrophoresis.

[33]  Amos Bairoch,et al.  Human liver protein map: A reference database established by microsequencing and gel comparison , 1992, Electrophoresis.