Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells.

UNLABELLED The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells. METHODS Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used. RESULTS The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness. CONCLUSION Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake.

[1]  S. Yefimova,et al.  Nano-scale liposomal container with a «signal system» for substances delivering in living cells , 2011 .

[2]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .

[3]  S. Yefimova,et al.  Effect of hydrophobicity of cationic carbocyanine dyes DiOCn on their binding to anionic surfactant micelles , 2010 .

[4]  Vincent M. Rotello,et al.  The biomacromolecule-nanoparticle interface , 2007 .

[5]  Joseph M. DeSimone,et al.  Strategies in the design of nanoparticles for therapeutic applications , 2010, Nature Reviews Drug Discovery.

[6]  R. Haugland.  Handbook of fluorescent probes and research products , 2002 .

[7]  M. Hope,et al.  Extrusion technique to generate liposomes of defined size. , 2003, Methods in enzymology.

[8]  B. Valeur,et al.  Molecular Fluorescence: Principles and Applications , 2001 .

[9]  Jian Ni,et al.  Applications of the MTT assay to functional studies of mouse intestinal intraepithelial lymphocytes , 1996, Journal of clinical laboratory analysis.

[10]  Kazunori Kataoka,et al.  Current state, achievements, and future prospects of polymeric micelles as nanocarriers for drug and gene delivery. , 2006, Pharmacology & therapeutics.

[11]  O. Katare,et al.  Liposomal drug delivery systems--clinical applications. , 2005, Acta pharmaceutica.

[12]  Kinam Park,et al.  Release of hydrophobic molecules from polymer micelles into cell membranes revealed by Förster resonance energy transfer imaging , 2008, Proceedings of the National Academy of Sciences.

[13]  A. Kozubek,et al.  Liposomal drug delivery, a novel approach: PLARosomes. , 2000, Acta biochimica Polonica.