Democratizing DNA Fingerprinting

We report a rapid, inexpensive, and portable strategy to re-identify human DNA using the MinION, a miniature sequencing sensor by Oxford Nanopore Technologies. Our strategy requires only 10-30 minutes of MinION sequencing, works with low input DNA, and enables familial searches. We also show that it can re-identify individuals from Direct-to-Consumer genomic datasets that are publicly available. We discuss potential forensic applications as well as the legal and ethical implications of a democratized DNA fingerprinting strategy available to the public.

[1]  R. Munzner,et al.  Regulatory issues , 2005, Alzheimer's & Dementia.

[2]  Stevan B. Jovanovich,et al.  Developmental validation studies on the RapidHIT™ Human DNA Identification System , 2013 .

[3]  Yaniv Erlich,et al.  Using mobile sequencers in an academic classroom , 2016, eLife.

[4]  Latanya Sweeney,et al.  Identifying Participants in the Personal Genome Project by Name , 2013, ArXiv.

[5]  Matthew Loose,et al.  Real-time selective sequencing using nanopore technology , 2016, Nature Methods.

[6]  Manfred Kayser,et al.  Improving human forensics through advances in genetics, genomics and molecular biology , 2011, Nature Reviews Genetics.

[7]  J. Molinuevo,et al.  Regulatory Issues , 2002 .

[8]  Euan A Ashley,et al.  A public resource facilitating clinical use of genomes , 2012, Proceedings of the National Academy of Sciences.

[9]  Benedict Paten,et al.  Improved data analysis for the MinION nanopore sequencer , 2015, Nature Methods.

[10]  Elizabeth E Joh Reclaiming "abandoned" DNA: the Fourth Amendment and genetic privacy. , 2006, Northwestern University law review.

[11]  Jason Nolan,et al.  Sousveillance: Inventing and Using Wearable Computing Devices for Data Collection in Surveillance Environments. , 2002 .

[12]  Yaniv Erlich A vision for ubiquitous sequencing , 2015, bioRxiv.

[13]  P. Bayer,et al.  openSNP–A Crowdsourced Web Resource for Personal Genomics , 2014, PloS one.

[14]  Zhen Lin,et al.  Genomic Research and Human Subject Privacy , 2004, Science.

[15]  Robert J. Fischer,et al.  Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool , 2016, Emerging infectious diseases.

[16]  Mick Watson,et al.  Successful test launch for nanopore sequencing , 2015, Nature Methods.

[17]  J. Zlotogora Genetics and genomic medicine in Israel , 2014, Molecular genetics & genomic medicine.

[18]  Bert-Jaap Koops,et al.  Forensic DNA Phenotyping: Regulatory Issues , 2006 .

[19]  Stephen E. Strauss,et al.  DNA goes to court , 2012, Nature Biotechnology.

[20]  David A. Eccles,et al.  MinION Analysis and Reference Consortium: Phase 1 data release and analysis , 2015, F1000Research.

[21]  David A. Matthews,et al.  Real-time, portable genome sequencing for Ebola surveillance , 2016, Nature.

[22]  Eran Halperin,et al.  Identifying Personal Genomes by Surname Inference , 2013, Science.

[23]  Timothy B. Stockwell,et al.  The Diploid Genome Sequence of an Individual Human , 2007, PLoS biology.

[24]  David Lazer,et al.  Finding Criminals Through DNA of Their Relatives , 2006, Science.

[25]  Sirpa Soini,et al.  Genetic testing legislation in Western Europe—a fluctuating regulatory target , 2012, Journal of Community Genetics.