Pathotypical Characterization and Molecular Epidemiology of Newcastle Disease Virus Isolates from Different Hosts in China from 1996 to 2005

ABSTRACT Thirty Newcastle disease virus (NDV) strains isolated from outbreaks in China during 1996 to 2005 were characterized pathotypically and genotypically. All strains except one were velogenic. An analysis of the variable region (nucleotides 47 to 420) of the F gene indicated that 6 isolates belonged to genotype II, 3 to genotype III, 1 (isolated from a pigeon) to genotype VI, and 20 to genotype VII. Isolates belonging to genotype VII were further divided into five subtypes, VIIa, VIIb, VIIc, VIId, and VIIe, and subtype VIId was made up of VIId1 to VIId5. These results showed that genotype VII isolates might have been the most prevalent in China during the past two decades. Genotype VII isolates shared high homology, but the homology was less than that between genotype VII viruses and the vaccine virus LaSota. Among these NDV isolates, 25 isolates had the velogenic motif 112R/K-R-Q-K/R-R-F117 that is consistent with results of the biological tests. However, four of five LaSota-type isolates that contained the lentogenic motif 112G-R-Q-G-R-L117 were velogenic, except SY/03, in the view of the biological test. The majority of genotype VII isolates had lost one or two N-glycosylation sites. Finally, a cross-protection experiment in which specific-pathogen-free chickens vaccinated with LaSota were challenged by six NDV isolates showed that more than three isolates were antigenic variants that could be responsible for recent outbreaks of Newcastle disease.

[1]  K. Handberg,et al.  Molecular Characterization and Phylogenetic Study of Newcastle Disease Virus Isolates from Recent Outbreaks in Eastern Uganda , 2004, Journal of Clinical Microbiology.

[2]  A. Burny,et al.  Mutations located on both F1 and F2 subunits of the Newcastle disease virus fusion protein confer resistance to neutralization with monoclonal antibodies , 1989, Journal of virology.

[3]  H. Wan,et al.  Pathotypical and genotypical characterization of strains of Newcastle disease virus isolated from outbreaks in chicken and goose flocks in some regions of China during 1985–2001 , 2003, Archives of Virology.

[4]  D. Alexander,et al.  Evaluation of the molecular basis of pathogenicity of the variant Newcastle disease viruses termed “pigeon PMV-1 viruses” , 2005, Archives of Virology.

[5]  J. Riva,et al.  Molecular Characterization of Avian Paramyxovirus 1 Isolates Collected from Cormorants in Canada from 1995 to 2000 , 2003, Journal of Clinical Microbiology.

[6]  K. Kuma,et al.  Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene. , 1989, Virology.

[7]  D. King,et al.  Molecular Characterization of Virulent Newcastle Disease Virus Isolates from Chickens during the 1998 NDV Outbreak in Kazakhstan , 2005, Virus Genes.

[8]  Y. Nagai,et al.  Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization , 1988, Journal of virology.

[9]  D. Higgins,et al.  See Blockindiscussions, Blockinstats, Blockinand Blockinauthor Blockinprofiles Blockinfor Blockinthis Blockinpublication Clustal: Blockina Blockinpackage Blockinfor Blockinperforming Multiple Blockinsequence Blockinalignment Blockinon Blockina Minicomputer Article Blockin Blockinin Blockin , 2022 .

[10]  S. B. Hitchner,et al.  A Laboratory Manual for the Isolation and Identification of Avian Pathogens , 1990 .

[11]  B. Seal,et al.  Use of a Heteroduplex Mobility Assay To Detect Differences in the Fusion Protein Cleavage Site Coding Sequence among Newcastle Disease Virus Isolates , 2001, Journal of Clinical Microbiology.

[12]  R. Álvarez,et al.  RNA-dependent RNA polymerase gene analysis of worldwide Newcastle disease virus isolates representing different virulence types and their phylogenetic relationship with other members of the paramyxoviridae. , 2004, Virus research.

[13]  K. Kuma,et al.  Newcastle disease virus evolution. II. Lack of gene recombination in generating virulent and avirulent strains. , 1989, Virology.

[14]  E. Somogyi,et al.  Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications. , 2006, Virus research.

[15]  J. Banks,et al.  A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene , 2003, Avian pathology : journal of the W.V.P.A.

[16]  J. Kwang,et al.  Characterization of Newly Emerging Newcastle Disease Virus Isolates from the People's Republic of China and Taiwan , 2001, Journal of Clinical Microbiology.

[17]  K. Yusoff,et al.  Location of neutralizing epitopes on the fusion protein of Newcastle disease virus strain Beaudette C. , 1989, The Journal of general virology.

[18]  L. Skoog,et al.  Historical aspects. , 2009, Monographs in clinical cytology.

[19]  B. Peeters,et al.  Rescue of Newcastle Disease Virus from Cloned cDNA: Evidence that Cleavability of the Fusion Protein Is a Major Determinant for Virulence , 1999, Journal of Virology.

[20]  N. Millar,et al.  Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. , 1986, The Journal of general virology.

[21]  B. Seal,et al.  Characterization of Newcastle disease virus isolates by reverse transcription PCR coupled to direct nucleotide sequencing and development of sequence database for pathotype prediction and molecular epidemiological analysis , 1995, Journal of clinical microbiology.

[22]  K. Shimokata,et al.  Expression of factor X and its significance for the determination of paramyxovirus tropism in the chick embryo. , 1992, The EMBO journal.

[23]  E. Kaleta,et al.  Newcastle disease outbreaks in recent years in Western Europe were caused by an old (VI) and a novel genotype (VII) , 1998, Archives of Virology.

[24]  Sudhir Kumar,et al.  MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment , 2004, Briefings Bioinform..

[25]  D. Alexander,et al.  Avian paramyxovirus type 1 infections of racing pigeons: 2 pathogenicity experiments in pigeons and chickens , 1984, Veterinary Record.