Cryopreservation of human platelets with dimethyl sulfoxide: changes in biochemistry and cell function

BACKGROUND: The shelf life of liquid‐stored platelet concentrates is limited to 5 days. Therefore, much work has been carried out in an attempt to establish the optimum method for cryopreservation. Among the various cryoprotectants, dimethyl sulfoxide (DMSO) has been shown to be the most effective. However, DMSO‐frozen platelets are characterized by a number of cell lesions. This report describes metabolic and functional changes that should give rise to some concern about the functional integrity of these cells.

[1]  J. Aisner,et al.  Frozen Autologous Platelets in the Supportive Care of Patients with Leukemia , 2003, Transfusion.

[2]  S. Holme,et al.  Membrane Glycoproteins in Cryopreserved Platelets , 1994, Vox sanguinis.

[3]  M. Heim,et al.  Storage of single‐donor platelet concentrates: metabolic and functional changes , 1993, Transfusion.

[4]  A. Berardi,et al.  Evaluation of Four Different Methods for Platelet Freezing: In vitro and in vivo Studies , 1992, Vox sanguinis.

[5]  M. Owens,et al.  Cryopreserved platelets have decreased adhesive capacity , 1991, Transfusion.

[6]  M. Taylor.  Cryopreservation of platelets: an in-vitro comparison of four methods. , 1981, Journal of clinical pathology.

[7]  C. Schiffer,et al.  Successful transfusion of platelets cryopreserved for more than 3 years. , 1979, Blood.

[8]  C. Valeri,et al.  Therapeutic Transfusions of Previously Frozen Washed Human Platelets , 1979, Transfusion.

[9]  C. Valeri,et al.  Ultrastructural Alterations and Phagocytic Function of Cryopreserved Platelets , 1979, Transfusion.

[10]  C. Valeri,et al.  Oxygen consumption, platelet aggregation and release reactions in platelets freeze‐preserved with dimethylsulfoxide , 1977, Transfusion.

[11]  C. Schiffer,et al.  Clinical Experience with Transfusion of Cryopreserved Platelets , 1976, British journal of haematology.

[12]  B. Kim,et al.  Storage of Human Platelets by Freezing 1 , 1976, Vox sanguinis.

[13]  C. Valeri,et al.  Changes in platelet shape and structure after freeze preservation. , 1974, Blood.

[14]  B. Kim,et al.  Biochemistry, Function, and Hemostatic Effectiveness of Frozen Human Platelets 1 , 1974, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[15]  G. Fioritoni,et al.  Evaluation of Four Different Methods for Platelet Freezing , 1992 .

[16]  N. Mulder,et al.  Bleeding prophylaxis in autologous bone marrow transplantation for solid tumors. Comparison of cryopreserved autologous and fresh allogeneic single-donor platelets. , 1989, Haemostasis.

[17]  M. Kratzer,et al.  Simulation of primary haemostasis in vitro. , 1985, Haemostasis.

[18]  L. Weintraub,et al.  Cryopreservation of human platelets using 6% dimethyl sulfoxide and storage at −80°C: effects of 2 years of frozen storage at −80°C and transportation in dry ice , 1985 .

[19]  C. Valeri,et al.  A Simple Method for Freezing Human Platelets Using 6% Dimethylsulfoxide and Storage at -80°C , 1974 .