Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin

A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V. cholerae non-O1 (strain A-5) by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatographies on SP-Sephadex C-50 and DEAE-Sephadex A-25, and high-pressure liquid chromatography on a Lichrosorb RP-8 column. About 1.4 X 10(5)-fold purification was achieved, with a recovery of about 12%. Although the crude preparation was heat labile, the purified toxin was heat stable. The minimum effective dose of purified toxin was about 5 ng in the suckling mouse assay. The amino acid composition of the purified toxin was determined to be Asp(3), Glu(1), Ala(1), half-Cys(6), Ile(2), Leu(1), Phe(1), and Pro(1). These data show the production of a new type of heat-stable enterotoxin (NAG-ST) by V. cholerae non-O1.

[1]  N. Tominaga,et al.  Primary structure of heat-stable enterotoxin produced by Yersinia enterocolitica. , 1984, Biochemical and biophysical research communications.

[2]  Y. Takeda,et al.  Evidence that a non-O1 Vibrio cholerae produces enterotoxin that is similar but not identical to cholera enterotoxin , 1983, Infection and immunity.

[3]  M. Nishibuchi,et al.  Vibrio factors cause rapid fluid accumulation in suckling mice , 1983, Infection and immunity.

[4]  Y. Takeda,et al.  Amino acid sequence of a heat‐stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D , 1983, FEBS letters.

[5]  M. Nishibuchi,et al.  Medium-dependent production of extracellular enterotoxins by non-O-1 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis , 1983, Applied and environmental microbiology.

[6]  Y. Takeda,et al.  Improved method for purification of heat-stable enterotoxin of Escherichia coli , 1983 .

[7]  Y. Takeda,et al.  Amino-acid sequence of a heat-stable enterotoxin produced by human enterotoxigenic Escherichia coli. , 1982, European journal of biochemistry.

[8]  Y. Takeda,et al.  Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment , 1981, Infection and immunity.

[9]  H. Lior,et al.  Cytotoxicity and suckling mouse reactivity of Aeromonas hydrophila isolated from human sources. , 1981, Canadian journal of microbiology.

[10]  W. Hill,et al.  Virulence of Three Clinical Isolates of Vibrio cholerae Non-O- 1 Serogroup in Experimental Enteric Infections in Rabbits , 1981, Infection and immunity.

[11]  P. Blake,et al.  Diseases of humans (other than cholera) caused by vibrios. , 1980, Annual review of microbiology.

[12]  Y. Takeda,et al.  Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli , 1979, Infection and immunity.

[13]  C. Pai,et al.  Production of enterotoxin by Yersinia enterocolitica , 1978, Infection and immunity.

[14]  R. Giannella.  Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model , 1976, Infection and immunity.

[15]  R. Sack,et al.  Human diarrheal disease caused by enterotoxigenic Escherichia coli. , 1975, Annual review of microbiology.

[16]  E. Aldová,et al.  Isolation of nonagglutinable vibrios from an enteritis outbreak in Czechoslovakia. , 1968, The Journal of infectious diseases.

[17]  J. Feeley,et al.  DIARRHEA CAUSED BY NON-CHOLERA VIBRIOS. , 1965, The American journal of tropical medicine and hygiene.