Clinical evaluation of serum type IV collagen 7S in idiopathic pulmonary fibrosis

Abstract Type IV collagen is one of the major components of the basement membrane (BM). 7S domain (7S collagen) of type IV collagen is an N‐terminal peptide which is stable against protease and heat. We investigated serum concentration of 7S collagen in patients with idiopathic pulmonary fibrosis (IPF) and other pulmonary diseases. The aim of this study was to evaluate whether changes in the serum concentration of 7S collagen reflect the fibrotic process of IPF. We measured the concentration of serum 7S collagen with radioimmunoassay in patients with IPF, chronic pulmonary emphysema (CPE), sarcoidosis, infectious pulmonary diseases (IPD) and normal healthy controls. We also monitored 7S collagen during the clinical course in some patients with IPF and investigated the correlation between the serum 7S collagen, and lactate dehydrogenase (LDH) and erthrocyte sedimentation rate (ESR) in patients with IPF. Patients with IPF showed significantly higher serum concentration of 7S collagen than other pulmonary diseases and healthy controls. The serum concentration of 7S collagen significantly decreased in IPF patients who showed roentgenographic improvement after corticosteroid treatment. There was a correlation between the serum 7S collagen and LDH, and ESR. In conclusion, serum concentrations of 7S collagen increase in patients with IPE The measurement of 7S collagen is useful for the evaluation of fibrotic change in the lung.

[1]  A. Engström‐Làurent,et al.  Serum aminoterminal type III procollagen peptide and the 7S domain of type IV collagen in patients with alcohol abuse. Relation to ultrastructural fibrosis in the acinar zone 3 and to serum hyaluronan. , 2008, Liver.

[2]  A. Ishizaka,et al.  Serum concentration of 7S collagen and prognosis in patients with the adult respiratory distress syndrome. , 1994, Thorax.

[3]  J. Risteli,et al.  Effect of age and diabetes on type IV collagen and laminin in human kidney cortex. , 1986, Kidney international.

[4]  J. Risteli,et al.  Type IV collagen and laminin‐related antigens in human serum in alcoholic liver disease , 1985, European journal of clinical investigation.

[5]  G. Raghu,et al.  Extracellular matrix in normal and fibrotic human lungs. , 1985, The American review of respiratory disease.

[6]  B. Voss,et al.  7 S collagen: a method for the measurement of serum concentrations in man. , 1984, Clinica chimica acta; international journal of clinical chemistry.

[7]  Y. Kanwar Biophysiology of glomerular filtration and proteinuria. , 1984, Laboratory investigation; a journal of technical methods and pathology.

[8]  P. Amenta,et al.  The basement membrane in pathology. , 1983, Laboratory investigation; a journal of technical methods and pathology.

[9]  R. Timpl,et al.  A network model for the organization of type IV collagen molecules in basement membranes. , 1981, European journal of biochemistry.

[10]  R. Timpl,et al.  Type IV collagens' isolation and characterization of 7S collagen from human kidney, liver and lung. , 1981, Collagen and related research.

[11]  R. Timpl,et al.  Macromolecular structure of basement membrane collagens , 1981, FEBS letters.

[12]  R. Timpl,et al.  Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes. , 1981, The Biochemical journal.

[13]  F. N. Low The extracellular portion of the human blood‐air barrier and its relation to tissue space , 1961 .

[14]  P H Bartels,et al.  Automated prescreening of conventionally prepared cervical smears: a feasibility study. , 1992, Laboratory investigation; a journal of technical methods and pathology.