Heteropolysaccharide Formation by Arthrobacter viscosus Grown on Xylose and Xylose Oligosaccharides

Arthrobacter viscosus NRRL B-1973 produces its viscous extracellular polysaccharides (EPS) when grown on media containing xylose or enzymatic xylan hydrolysates. Crude EPS formation from xylose averaged 12 g/liter when initial culture pH was adjusted to 8.0 and total nitrogen was limited to 0.03%. Purified EPS from pentose and hexose substrates were analyzed for their monosaccharide, acetyl, and uronic acid components, intrinsic viscosities, and average molecular masses. Differences were apparent in degrees of acetylation, molecular masses, and intrinsic viscosities of the heteropolysaccharides produced on different carbon sources.

[1]  V. Ginsburg.  SUGAR NUCLEOTIDES AND THE SYNTHESIS OF CARBOHYDRATES. , 1964, Advances in enzymology and related subjects of biochemistry.

[2]  P. Watson,et al.  Extracellular polysaccharide produced from glucose by Arthrobacter viscosus NRRL B-1973: Chemical and physical characterization† , 1965 .

[3]  D. Doherty,et al.  Genetic Engineering of Polysaccharide Structure: Production of Variants of Xanthan Gum in Xanthomonas campestris , 1990, Biotechnology progress.

[4]  J. Linton.  The relationship between metabolite production and the growth efficiency of the producing organism. , 1990, FEMS microbiology reviews.

[5]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[6]  J. Wells.  Extracellular Microbial Polysaccharides—A Critical Overview , 1977 .

[7]  K. Burton.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. , 1956, The Biochemical journal.

[8]  R. Mccready,et al.  Determination of Acetyl in Pectin and in Acetylated Carbohydrate Polymers , 1957 .

[9]  M. Rinaudo,et al.  Influence of acetyl and pyruvate contents on rheological properties of xanthan in dilute solution , 1987 .

[10]  N. Blumenkrantz,et al.  New method for quantitative determination of uronic acids. , 1973, Analytical biochemistry.

[11]  N. Takahashi,et al.  The major pectic arabinogalactan having activity on the reticuloendothelial system from the roots and rhizomes of Saposhnikovia divaricata. , 1989, Chemical & pharmaceutical bulletin.

[12]  F. Smith,et al.  Colorimetric Method for Determination of Sugars and Related Substances , 1956 .

[13]  J. BeMiller.  Industrial Polysaccharides: Genetic Engineering, Structure/Property Relations and Applications : Edited by Manssur Yalpani, Elsevier Science Publishers, Amsterdam, or P.O. Box 1663, Grand Central Station, New York, NY 10163 in North America, 1987, xix + 408 pages, $131.75, Dfl. 270.00 , 1988 .

[14]  A. Jeanes,et al.  A new modification of the carbazole analysis: application to heteropolysaccharides. , 1968, Analytical biochemistry.

[15]  M. I. T A I T,et al.  Effect of Growth Conditions on the Production , Composition and Viscosity of Xanthomonas campestris Exopolysaccharide , 2022 .

[16]  I. Sutherland.  Surface carbohydrates of the prokaryotic cell , 1977 .

[17]  G. Fuller,et al.  Rheologically interesting polysaccharides from yeasts , 1989, Applied biochemistry and biotechnology.

[18]  J. Touchstone.  Practice of Thin Layer Chromatography , 1978 .

[19]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[20]  J. P. Nakas,et al.  Purification and characterization of two xylanases from Trichoderma longibrachiatum. , 1991, European journal of biochemistry.

[21]  Fred W. Billmeyer,et al.  Experiments in Polymer Science , 1973 .

[22]  A. H. Rose.  Primary products of metabolism , 1978 .

[23]  P A Sandford.  Exocellular, microbial polysaccharides. , 1979, Advances in carbohydrate chemistry and biochemistry.

[24]  P. Watson,et al.  Structure of the extracellular bacterial polysaccharide from Arthrobacter viscosus NRRL B-1973 , 1968 .

[25]  J. Robyt,et al.  Biochemical Techniques: Theory and Practice , 1987 .

[26]  M. Cadmus,et al.  NEW BACTERIAL POLYSACCHARIDE FROM ARTHROBACTER. , 1963, Applied microbiology.