Nutritive and functional composition in different parts(body wall,intestine and spawn) of Stichopus japonicus were investigated in this paper.The results were shown as follows: Stichopus japonicus was of high protein and the content of protein in the intestine and spawn were higher than those in body wall;Cu,Fe,Mn,Zn and other metal elements were abundant,which were more likely enrichment in the intestine;the content of δ-VE was high,especially in the intestine;the percentage content of acid mucopolysaccharide in the body wall was high and the content was 5.03%;composition of amino acids was complete,and free amino acids(FAA) were rich.Hydroxyproline which was the basis of collagen protein accounted for 4.20% in the body wall;there were 23 kinds of fatty acids were detected and it was found that the essential fatty acids content was high,especially the content of C20∶5 n3(EPA) was 16.06% in the intestine.In conclusion,Stichopus japonicus was of high nutrition value and some officinal value.