Advances in Boar Semen Cryopreservation

The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI had been promoted. Recent advances in our laboratory, accommodating the best use of portions of the sperm-rich fraction of the ejaculate for cryopreservation of the sperm-peak portion (P1) and parallel use of the rest of the collected ejaculated spermatozoa, appears as a suitable commercial alternative.

[1]  H. Rodríguez-Martínez,et al.  Freezing of boar semen can be simplified by handling a specific portion of the ejaculate with a shorter procedure and MiniFlatPack packaging. , 2010, Animal reproduction science.

[2]  H. Rodríguez-Martínez,et al.  The physiological roles of the boar ejaculate. , 2020, Society of Reproduction and Fertility supplement.

[3]  H. Rodríguez-Martínez,et al.  Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks. , 2009, Theriogenology.

[4]  H. Rodríguez-Martínez,et al.  Effects of straw volume and Equex-STM on boar sperm quality after cryopreservation. , 2009, Reproduction in domestic animals = Zuchthygiene.

[5]  H. Ekwall Cryo-scanning electron microscopy discloses differences in dehydration of frozen boar semen stored in large containers. , 2009, Reproduction in domestic animals = Zuchthygiene.

[6]  Zhong-liang Jiang,et al.  Effects of different extenders on DNA integrity of boar spermatozoa following freezing-thawing. , 2008, Cryobiology.

[7]  H. Rodríguez-Martínez,et al.  Influence of seminal plasma on the kinematics of boar spermatozoa during freezing. , 2008, Theriogenology.

[8]  E. M. Madeira,et al.  Evaluation of amides and centrifugation temperature in boar semen cryopreservation. , 2008, Theriogenology.

[9]  H. Rodríguez-Martínez,et al.  Controlled cooling during semen cryopreservation does not induce capacitation of spermatozoa from two portions of the boar ejaculate. , 2007, International journal of andrology.

[10]  F. Fonseca,et al.  Rapidly cooled horse spermatozoa: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation. , 2007, Theriogenology.

[11]  Leyland Fraser,et al.  Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing. , 2007, Animal reproduction science.

[12]  H. Rodríguez-Martínez,et al.  Cryo-scanning electron microscopy (Cryo-SEM) of boar semen frozen in medium-straws and MiniFlatPacks. , 2007, Theriogenology.

[13]  J. Roca,et al.  Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability. , 2007, Theriogenology.

[14]  Shu-shan Zhang,et al.  Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay. , 2007, Animal reproduction science.

[15]  G. Evans,et al.  Effects of platelet-activating factor and platelet-activating factor: acetylhydrolase on in vitro post-thaw boar sperm parameters. , 2007, Theriogenology.

[16]  M. Hernandez,et al.  Factors influencing boar sperm cryosurvival. , 2006, Journal of animal science.

[17]  G. J. Morris Rapidly cooled human sperm: no evidence of intracellular ice formation. , 2006, Human reproduction.

[18]  F. Fonseca,et al.  The high viscosity encountered during freezing in glycerol solutions: effects on cryopreservation. , 2006, Cryobiology.

[19]  H. Rodríguez-Martínez,et al.  Fertility after deep intra-uterine artificial insemination of concentrated low-volume boar semen doses. , 2006, Theriogenology.

[20]  Szabolcs Nagy,et al.  Deep freezing of concentrated boar semen for intra-uterine insemination: effects on sperm viability. , 2005, Theriogenology.

[21]  H. Woelders,et al.  Cryopreservation of boar semen: equilibrium freezing in the cryomicroscope and in straws. , 2005, Theriogenology.

[22]  J. Roca,et al.  Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. , 2005, Journal of andrology.

[23]  H. Woelders,et al.  Theoretical prediction of 'optimal' freezing programmes. , 2004, Cryobiology.

[24]  B. Fuller,et al.  Cryoprotectants: the essential antifreezes to protect life in the frozen state. , 2004, Cryo letters.

[25]  F. Peña,et al.  Antioxidant supplementation of boar spermatozoa from different fractions of the ejaculate improves cryopreservation: changes in sperm membrane lipid architecture , 2004, Zygote.

[26]  R. Devireddy,et al.  Subzero water transport characteristics of boar spermatozoa confirm observed optimal cooling rates , 2004, Molecular reproduction and development.

[27]  J. Bischof,et al.  Thermodynamic nonequilibrium phase change behavior and thermal properties of biological solutions for cryobiology applications. , 2004, Journal of biomechanical engineering.

[28]  H. Rodríguez-Martínez,et al.  Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation. , 2004, Theriogenology.

[29]  F. Peña,et al.  Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. , 2003, Animal reproduction science.

[30]  Lisa M Thurston,et al.  Post-thaw functional status of boar spermatozoa cryopreserved using three controlled rate freezers: a comparison. , 2003, Theriogenology.

[31]  G. Im,et al.  Lactose-egg yolk diluent supplemented with N-acetyl-D-glucosamine affect acrosome morphology and motility of frozen-thawed boar sperm. , 2002, Animal reproduction science.

[32]  H. Rodríguez-Martínez,et al.  Field fertility with exported boar semen frozen in the new flatpack container. , 2002, Theriogenology.

[33]  Y. Yi,et al.  Effect of N-acetyl-D-glucosamine, and glycerol concentration and equilibration time on acrosome morphology and motility of frozen-thawed boar sperm. , 2002, Animal reproduction science.

[34]  H. Rodríguez-Martínez,et al.  Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa frozen in FlatPacks and Maxi-straws. , 2000, Animal reproduction science.

[35]  K. Weitze,et al.  Storage of boar semen. , 2000, Animal reproduction science.

[36]  H. Rodríguez-Martínez,et al.  Deep-freezing of boar semen in plastic film 'cochettes'. , 2000, Journal of veterinary medicine. A, Physiology, pathology, clinical medicine.

[37]  B. Eriksson Cryopreservation of boar semen , 2000 .

[38]  H. Rodríguez-Martínez,et al.  Membrane damage during dilution, cooling and freezing-thawing of boar spermatozoa packaged in plastic bags. , 1994, Zentralblatt fur Veterinarmedizin. Reihe A.

[39]  H. Rodríguez-Martínez,et al.  Deep Freezing of Boar Semen Packaged in Plastic Bags and Straws , 1991 .

[40]  C. Bwanga Cryopreservation of boar semen. I: A literature review. , 1991, Acta veterinaria Scandinavica.

[41]  H. Rodríguez-Martínez,et al.  Cryopreservation of boar semen. III: Ultrastructure of boar spermatozoa frozen ultra-rapidly at various stages of conventional freezing and thawing. , 1991, Acta veterinaria Scandinavica.

[42]  D. Rath,et al.  [New aspects of preservation of boar sperm by deep freezing in plastic tubes]. , 1987, DTW. Deutsche tierarztliche Wochenschrift.

[43]  Takahiko Kato Enzymatic microdetermination method for choline acetyltransferase , 1983 .

[44]  H. Moore,et al.  Fertility of boar spermatozoa after freezing in the absence of seminal vesicular proteins. , 1977, Journal of reproduction and fertility.