Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods

We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.

[1]  L De Vuyst,et al.  The role and application of enterococci in food and health. , 2006, International journal of food microbiology.

[2]  G. Bou,et al.  Lack of correlation between phenotypic techniques and PCR-based genotypic methods for identification of Enterococcus spp. , 2004, Diagnostic microbiology and infectious disease.

[3]  L. Winston,et al.  API 20 strep identification system may incorrectly speciate enterococci with low level resistance to vancomycin. , 2004, Diagnostic microbiology and infectious disease.

[4]  W. Kneifel,et al.  Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp. 1. Media for isolation and enumeration. , 2003, International journal of food microbiology.

[5]  W. Kneifel,et al.  Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp. 2. Pheno- and genotypic criteria. , 2003, International journal of food microbiology.

[6]  S. Mpuchane,et al.  Enterococcus faecalis and Enterococcus faecium isolates from milk, beef, and chicken and their antibiotic resistance. , 2003, Journal of food protection.

[7]  G. Giraffa,et al.  Enterococci from foods. , 2002, FEMS microbiology reviews.

[8]  M. Gasson,et al.  Molecular Screening of EnterococcusVirulence Determinants and Potential for Genetic Exchange between Food and Medical Isolates , 2001, Applied and Environmental Microbiology.

[9]  F. Baquero,et al.  Vancomycin-resistant enterococci isolated from animals and food. , 2000, International journal of food microbiology.

[10]  Ronald N. Jones,et al.  Practical approach to the identification of clinically relevant Enterococcus species. , 1999, Diagnostic microbiology and infectious disease.

[11]  B. Cookson,et al.  Enterococci as emerging pathogens of humans , 1997, Journal of applied microbiology.

[12]  L. van Damme,et al.  Identification of Enterococcus species isolated from foods of animal origin. , 1995, International journal of food microbiology.

[13]  P. Courvalin,et al.  Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR , 1995, Journal of clinical microbiology.

[14]  M. Collins,et al.  Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. , 1993, The Journal of applied bacteriology.