Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

[1]  M. Goumans,et al.  Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation. , 2017, Nanomedicine : nanotechnology, biology, and medicine.

[2]  E. Wouters,et al.  Cigarette smoke extract induced exosome release is mediated by depletion of exofacial thiols and can be inhibited by thiol‐antioxidants , 2017, Free Radical Biology & Medicine.

[3]  P. Savelkoul,et al.  Bead-based flow-cytometry for semi-quantitative analysis of complex membrane vesicle populations released by bacteria and host cells. , 2017, Microbiological research.

[4]  I. Miinalainen,et al.  Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research , 2017, Scientific Reports.

[5]  M. Vidal,et al.  New insights into the function of Rab GTPases in the context of exosomal secretion , 2017, Small GTPases.

[6]  D. Meckes,et al.  Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers , 2016, Oncotarget.

[7]  A. Hill,et al.  Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey , 2016, Journal of extracellular vesicles.

[8]  Z. Giricz,et al.  Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods , 2015, PloS one.

[9]  Qihong Huang,et al.  Telomeric repeat-containing RNA (TERRA) constitutes a nucleoprotein component of extracellular inflammatory exosomes , 2015, Proceedings of the National Academy of Sciences.

[10]  Ching-Seng Ang,et al.  FunRich: An open access standalone functional enrichment and interaction network analysis tool , 2015, Proteomics.

[11]  Henrik J Johansson,et al.  Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. , 2015, Nanomedicine : nanotechnology, biology, and medicine.

[12]  Jan Lötvall,et al.  EVpedia: A community web resource for prokaryotic and eukaryotic extracellular vesicles research. , 2015, Seminars in cell & developmental biology.

[13]  A. Brisson,et al.  High-speed centrifugation induces aggregation of extracellular vesicles , 2015, Journal of extracellular vesicles.

[14]  H. D. del Portillo,et al.  Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals , 2015, Journal of extracellular vesicles.

[15]  L. O’Driscoll,et al.  Biological properties of extracellular vesicles and their physiological functions , 2015, Journal of extracellular vesicles.

[16]  Gladys N. Nangami,et al.  Fetuin-A associates with histones intracellularly and shuttles them to exosomes to promote focal adhesion assembly resulting in rapid adhesion and spreading in breast carcinoma cells. , 2014, Experimental cell research.

[17]  T. Best,et al.  Interrelated role of cigarette smoking, oxidative stress, and immune response in COPD and corresponding treatments. , 2014, American journal of physiology. Lung cellular and molecular physiology.

[18]  Andrew F. Hill,et al.  Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles , 2014, Journal of extracellular vesicles.

[19]  J. Lötvall,et al.  The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles , 2014, Journal of extracellular vesicles.

[20]  R. Nieuwland,et al.  Single-step isolation of extracellular vesicles by size-exclusion chromatography , 2014, Journal of extracellular vesicles.

[21]  Aled Clayton,et al.  How pure are your vesicles? , 2013, Journal of extracellular vesicles.

[22]  J. Lötvall,et al.  EVpedia: an integrated database of high-throughput data for systemic analyses of extracellular vesicles , 2013, Journal of extracellular vesicles.

[23]  E. Kroh,et al.  Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma , 2011, Proceedings of the National Academy of Sciences.

[24]  J. Thyberg,et al.  Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid , 2003, European Respiratory Journal.

[25]  Magnus Östberg,et al.  Apparent pore size distributions of chromatography media , 1996 .

[26]  P. Wolf.  The Nature and Significance of Platelet Products in Human Plasma , 1967, British journal of haematology.