FLAVIN TYPE ACTION SPECTRUM OF NITRATE UTILIZATION BY Monoraphidium braunii

The light‐dependent utilization of nitrate by the green alga Monoraphidium braunii, coming from nocturnal dark periods, shows an action spectrum of flavin type with two main bands: one in the blue, peaking at 450 and 480 nm, and the other in the near‐UV region with a maximum at 365 nm. Other results indicate that cells growing on nitrate as the only nitrogen source resynthesize nitrate reductase daily, which implies the nocturnal loss of this enzyme. The biosynthesis of nitrate reductase at the beginning of the light periods can proceed under red light. In addition, blue or near‐UV light is required for the activation of the previously formed nitrate reductase.

[1]  M. Quiñones,et al.  Blue Light Activation of Nitrate Reductase and Blue Light Promotion of the Biosynthesis of Nitrite Reductase in Monoraphidium Braunii , 1990 .

[2]  J. Whitaker,et al.  Synthesis and degradation of nitrate reductase during the cell cycle of Chlorella sorokiniana. , 1989, Plant physiology.

[3]  J. Maldonado,et al.  PHOTOREGULATION OF NITRATE UTILIZATION IN GREEN ALGAE AND HIGHER PLANTS * , 1985 .

[4]  P. Aparicio,et al.  Spectral Dependence of Photoregulation of Inorganic Nitrogen Metabolism in Chlamydomonas reinhardii. , 1985, Plant physiology.

[5]  P. Song.  Photophysical Aspects of Blue Light Receptors: The Old Question (Flavins Versus Carotenoids) Re-Examined , 1984 .

[6]  P. Aparicio,et al.  Wavelength Dependence of Nitrite Release and the Effects of Different Nitrogen Sources and C0 2 Tensions on Chlamydomonas reinhardii Inorganic Nitrogen Metabolism , 1984 .

[7]  H. Senger.  Cryptochrome, Some Terminological Thoughts , 1984 .

[8]  J. Maldonado,et al.  Blue-light reactivation of spinach nitrate reductase inactivated by acetylene or cyanide. Effects of flavins and oxygen , 1983 .

[9]  P. Aparicio,et al.  In Vivo Blue-Light Activation of Chlamydomonas reinhardii Nitrate Reductase. , 1983, Plant physiology.

[10]  W. Ullrich.  Uptake and Reduction of Nitrate: Algae and Fungi , 1983 .

[11]  M. Losada,et al.  The Assimilatory Reduction of Nitrate , 1981 .

[12]  W. L. Butler,et al.  PHOTOACTIVATION OF NITRATE REDUCTASE FROM NEUROSPORA CRASSA , 1980 .

[13]  F. Calero,et al.  Blue light photoreactivation of nitrate reductase from green algae and higher plants. , 1976, Biochemical and biophysical research communications.

[14]  F. D. Snell,et al.  Colorimetric Methods of Analysis , 1945 .