Multiple Signals Regulate Nicotine Synthesis in Tobacco Plant

After wounding of tobacco plants, roots synthesize a large amount of nicotine to be transported to the shoot. Jasmonic acid (JA) acts as a long-distance signal between the wounding stimulus and response in tobacco plants. In addition, another phloem-mobile signal (auxin) plays a role in the transmission of the message triggered by wounding. Auxin can serve as a negative signal to regulate nicotine synthesis in roots of tobacco plants, even when plants are not wounded. Furthermore, removing the shoot apex after girdling the stem base still stimulates nicotine synthesis in roots. Since girdling prevented the involvement of signals transported in the phloem, this wound likely induced a response of nicotine synthesis in roots regulated by a signal transported via an alternative pathway. The results suggest that there are multiple signals in tobacco plant to regulate nicotine synthesis, depending on the treatment.

[1]  Andrzej Tretyn,et al.  Acetylcholine in plants: Presence, metabolism and mechanism of action , 2008, The Botanical Review.

[2]  Fusuo Zhang,et al.  Nicotine synthesis in Nicotiana tabacum L. induced by mechanical wounding is regulated by auxin. , 2006, Journal of experimental botany.

[3]  Rainer Stahlberg,et al.  Historical Overview on Plant Neurobiology , 2006, Plant signaling & behavior.

[4]  Rainer Stahlberg,et al.  Slow Wave Potentials — a Propagating Electrical Signal Unique to Higher Plants , 2006 .

[5]  J. D. Rhodes,et al.  Signals and Signalling Pathways in Plant Wound Responses , 2006 .

[6]  František Baluška,et al.  Neurobiological View of Plants and Their Body Plan , 2006 .

[7]  I. Baldwin,et al.  Nicotine's Defensive Function in Nature , 2004, PLoS biology.

[8]  Yasuyuki Yamada,et al.  Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures , 1998, Plant Molecular Biology.

[9]  I. Baldwin,et al.  Plant responses to insect herbivory: the emerging molecular analysis. , 2002, Annual review of plant biology.

[10]  C. Lou.  Integrated Action in Plant Irritability , 1998 .

[11]  P. Gantet,et al.  Necessity of a Functional Octadecanoic Pathway for Indole Alkaloid Synthesis by Catharanthus roseus Cell Suspensions Cultured in an Auxin-Starved Medium , 1998 .

[12]  K. Nakamura,et al.  An mRNA of tobacco cell, which is rapidly inducible by methyl jasmonate in the presence of cycloheximide, codes for a putative glycosyltransferase. , 1998, Plant & cell physiology.

[13]  I. Baldwin,et al.  Transport of [2-14C]jasmonic acid from leaves to roots mimics wound-induced changes in endogenous jasmonic acid pools in Nicotiana sylvestris , 1997, Planta.

[14]  I. Baldwin,et al.  Within-plant relationships among wounding, jasmonic acid, and nicotine implications for defence in Nicotiana sylvestris. , 1997, The New phytologist.

[15]  I. Baldwin,et al.  Quantification, correlations and manipulations of wound-induced changes in jasmonic acid and nicotine in Nicotiana sylvestris , 1997, Planta.

[16]  M. Malone,et al.  Wound-induced hydraulic signals and stimulus transmission in Mimosa pudica L. , 1994, The New phytologist.

[17]  J. Mullet,et al.  Sucrose Modulation of Soybean Vsp Gene Expression Is Inhibited by Auxin , 1994, Plant physiology.

[18]  R. Creelman,et al.  Coregulation of soybean vegetative storage protein gene expression by methyl jasmonate and soluble sugars. , 1992, Plant physiology.

[19]  J. Mullet,et al.  Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. , 1990, The Plant cell.