Glycolipid Biosurfactant from Indigenous Pseudomonas sps. isolated from Kandigai, Kanchipuram District For the Removal of Cr(III)

Heavy metal contaminants in fresh water is a major cause for most health issues and remediation of these contaminants are quite a tedious process but with the help of biosurfactants produced by bacteria isolated from soil, these contaminants can be removed in an effective manner. Chromium(III) is one of the heavy metal contaminants in fresh water. Experimental samples were prepared in laboratory containing chromium(III) at various concentrations (100ppm, 200 ppm,300,400 ppm,500 ppm) and it is treated with biosurfactant produced by bacteria isolated from soil from Kandigai, Kanchipuram district of Tamil Nadu. Biosurfactant producing bacteria were characterized by phenotypic methods and was identified based on Bergy’smanual and it was identified as Pseudomonas sps. Biosurfactant was characterized by oil displacement method,phenol:chloroform qualitative test, protein estimation, carbohydrate estimation, SDS-PAGE and FTIR analysis. Based on these studies, the biosurfactant was characterized as glycolipids. The same biosurfactant was used to remove Cr(III) from aqueous system and 89.3% of 100ppm/lt Cr(III) was removed in 10 minutes at 120rpm by 10mg of biosurfactant. The current research proves that this glycolipid biosurfactant produced by the indigenous bacteria could be effectively used to remove chromium(III) from aquatic environments.