Holographic optical tweezers in the Fresnel regime

We present a flexible setup for steering of laser tweezers using a high resolution spatial light modulator (SLM). Moving of e.g. trapped cells in the focal plane of the microscope objective is possible without the need for time consuming re-calculation of holograms. Numerous light spots or other modes like the so called "doughnut modes", which carry angular momentum, can be created and controlled independently by "mouse-dragging" the hologram window at the SLM display. In addition, undesired diffraction orders are suppressed using adequately calculated fresnel holograms.

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