Genome Sequence of a Heterocystous Diazotroph Isolated from a Trichodesmium Consortium from the North Pacific Subtropical Gyre

Diazotrophic cyanobacteria play a vital role in the nitrogen influx of the global marine ecosystem. In July 2010, colonies of Trichodesmium spp. were picked near Station ALOHA in the oligotrophic North Pacific Subtropical Gyre, and a novel heterocystous diazotroph (strain HetDA_MAG_MS3) belonging to the genus Rivularia was found living in close association; it was cultured and sequenced. ABSTRACT Diazotrophic cyanobacteria play a vital role in the nitrogen influx of the global marine ecosystem. In July 2010, colonies of Trichodesmium spp. were picked near Station ALOHA in the oligotrophic North Pacific Subtropical Gyre, and a novel heterocystous diazotroph (strain HetDA_MAG_MS3) belonging to the genus Rivularia was found living in close association; it was cultured and sequenced.

[1]  Christopher J Neely,et al.  MetaSanity: an integrated microbial genome evaluation and annotation pipeline , 2020, Bioinform..

[2]  Donovan H Parks,et al.  GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database , 2019, Bioinform..

[3]  Feng Li,et al.  MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies , 2019, PeerJ.

[4]  P. Pevzner,et al.  metaSPAdes: a new versatile metagenomic assembler. , 2017, Genome research.

[5]  Alexander J Probst,et al.  Recovery of genomes from metagenomes via a dereplication, aggregation and scoring strategy , 2017, Nature Microbiology.

[6]  Elaina D. Graham,et al.  BinSanity: unsupervised clustering of environmental microbial assemblies using coverage and affinity propagation , 2016, bioRxiv.

[7]  Connor T. Skennerton,et al.  CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes , 2015, Genome research.

[8]  Patrick K. H. Lee,et al.  A novel cohabitation between two diazotrophic cyanobacteria in the oligotrophic ocean , 2014, The ISME Journal.

[9]  Anders F. Andersson,et al.  Binning metagenomic contigs by coverage and composition , 2014, Nature Methods.

[10]  Björn Usadel,et al.  Trimmomatic: a flexible trimmer for Illumina sequence data , 2014, Bioinform..

[11]  Steven L Salzberg,et al.  Fast gapped-read alignment with Bowtie 2 , 2012, Nature Methods.

[12]  J. Rochaix.  Regulation of photosynthetic electron transport. , 2011, Biochimica et biophysica acta.

[13]  F. Tabita,et al.  Function, Structure, and Evolution of the RubisCO-Like Proteins and Their RubisCO Homologs , 2007, Microbiology and Molecular Biology Reviews.

[14]  Savita Singh.  Cyanobacteria and Their Role Under Elevated CO2 Conditions , 2019, Climate Change and Agricultural Ecosystems.

[15]  E. H. Harris,et al.  Organellar and metabolic processes , 2009 .

[16]  M. Spalding,et al.  Chapter 4 – Amino Acid Metabolism , 2009 .