An integrated membrane system for the biocatalytic production of 3'-sialyllactose from dairy by-products.

An integrated membrane system was investigated for the production of 3'-sialyllactose by an engineered sialidase using casein glycomacropeptide (CGMP) and lactose as substrates. CGMP was purified by ultrafiltration (UF) to remove any small molecules present and then an enzymatic membrane reactor (EMR) was used to separate the product and reuse the enzyme. A PLCC regenerated cellulose membrane was found to be the most suitable for both the UF purification and EMR. Subsequently, nanofiltration (NF) was conducted to increase the purity of the 3'-sialyllactose by removing the excess lactose present. The NTR7450 membrane outperformed others in NF due to its high retention of 3'-sialyllactose (98%) and relatively low rejection of lactose (40%). The lactose in the permeate could be concentrated by the NF45 membrane and recycled into the EMR. The described integrated membrane system enables a more economic and efficient enzymatic production of 3'-sialyllactose.

[1]  P. Gopal,et al.  Oligosaccharides and glycoconjugates in bovine milk and colostrum , 2000, British Journal of Nutrition.

[2]  L. Giorno,et al.  Purification of triacylglycerols for biodiesel production from Nannochloropsis microalgae by membrane technology. , 2013, Bioresource technology.

[3]  G. Foley.  Water usage in variable volume diafiltration: comparison with ultrafiltration and constant volume diafiltration , 2006 .

[4]  M. Vincent-Vela,et al.  Nanofiltration of sweet whey and prediction of lactose retention as a function of permeate flux using the Kedem–Spiegler and Donnan Steric Partioning models , 2007 .

[5]  A. Frasch,et al.  A sialidase mutant displaying trans-sialidase activity. , 2005, Journal of molecular biology.

[6]  R D Appel,et al.  Protein identification and analysis tools in the ExPASy server. , 1999, Methods in molecular biology.

[7]  Matthias Wessling,et al.  Membrane-based recovery of glucose from enzymatic hydrolysis of ionic liquid pretreated cellulose. , 2013, Bioresource technology.

[8]  J. Boza,et al.  Goats' milk as a natural source of lactose-derived oligosaccharides: Isolation by membrane technology , 2006 .

[9]  K. Fukuda,et al.  Recent Advances in Studies on Milk Oligosaccharides of Cows and Other Domestic Farm Animals , 2013, Bioscience, biotechnology, and biochemistry.

[10]  M. Mänttäri,et al.  Performance of RC and PES ultrafiltration membranes in filtration of pulp mill process waters , 2010 .

[11]  L. Ozimek,et al.  Isolation and analysis of κ-casein glycomacropeptide from goat sweet whey , 2002 .

[12]  A. Meyer,et al.  Optimizing the biocatalytic productivity of an engineered sialidase from Trypanosoma rangeli for 3'-sialyllactose production. , 2014, Enzyme and microbial technology.

[13]  Neelima,et al.  Chemical and functional properties of glycomacropeptide (GMP) and its role in the detection of cheese whey adulteration in milk: a review , 2013, Dairy science & technology.

[14]  A. Pilosof,et al.  Casein glycomacropeptide pH-dependent self-assembly and cold gelation , 2010 .

[15]  Vicki Chen,et al.  Ultrafiltration of protein mixtures: measurement of apparent critical flux, rejection performance, and identification of protein deposition , 2002 .

[16]  Anne S. Meyer,et al.  Biocatalytic production of 3′-sialyllactose by use of a modified sialidase with superior trans-sialidase activity , 2014 .

[17]  Todd J. Menkhaus,et al.  Recovery of high value protein from a corn ethanol process by ultrafiltration and an exploration of , 2011 .

[18]  Jianquan Luo,et al.  Effects of pH and salt on nanofiltration—a critical review , 2013 .

[19]  C. Wan,et al.  Separate and Concentrate Lactic Acid Using Combination of Nanofiltration and Reverse Osmosis Membranes , 2008, Applied biochemistry and biotechnology.

[20]  Jianquan Luo,et al.  Application of ultrafiltration and nanofiltration for recycling cellulase and concentrating glucose from enzymatic hydrolyzate of steam exploded wheat straw. , 2012, Bioresource technology.

[21]  Jesper Holck,et al.  Enzyme catalysed production of sialylated human milk oligosaccharides and galactooligosaccharides by Trypanosoma cruzi trans-sialidase. , 2014, New biotechnology.

[22]  M. El-Salam.  Separation of Casein Glycomacropeptide from Whey: Methods of Potential Industrial Application , 2006 .

[23]  R. Hickey.  The role of oligosaccharides from human milk and other sources in prevention of pathogen adhesion , 2012 .

[24]  M. Jaffrin,et al.  A two-stage ultrafiltration and nanofiltration process for recycling dairy wastewater. , 2011, Bioresource technology.

[25]  Yinghong Gao,et al.  The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion , 1998, Nature Biotechnology.