Quantification of feline immunodeficiency virus (FIV) proviral DNA in peripheral blood mononuclear cells of cats infected with Japanese strains of FIV.

The polymerase chain reaction (PCR) method was applied for measurement of the proviral DNA copy number of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMC) of cats experimentally and naturally infected with FIV. In experimentally infected cats except one cat infected with the Petaluma strain, FIV-specific DNAs were efficiently amplified with the PCR method under the conditions used in this study. In the naturally FIV-infected cats, the specific DNAs were also amplified. We established a quantitative method for measurement of proviral DNA copy number in PBMC from cats infected with TM2-type of FIV strains, and found that the number was variable among the six cats examined, ranging from 10(4.0) to 10(5.7) copies per 10(5) PBMCs. This method can be applicable to cats naturally infected with FIV of TM2-type. Proviral DNA quantitation developed here could be useful as an additional parameter to evaluate the relationships among the proviral load, immune response and development of the clinical symptoms, and to monitor efficacy of antiviral therapy in vivo.

[1]  E. G. Shpaer,et al.  Identification of three feline immunodeficiency virus (FIV) env gene subtypes and comparison of the FIV and human immunodeficiency virus type 1 evolutionary patterns , 1994, Journal of virology.

[2]  Calvin M. Johnson,et al.  FIV as a Model for AIDS Vaccination , 1994 .

[3]  J. Meers,et al.  The detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction. , 1993, Veterinary microbiology.

[4]  T. Mikami,et al.  Biological nature of feline immunodeficiency virus. , 1993, The Journal of veterinary medical science.

[5]  A. Hasegawa,et al.  Detection of feline immunodeficiency virus proviral DNA in feline peripheral blood mononuclear cells by the nested two-step polymerase chain reaction. , 1993, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[6]  L. L. Nesse,et al.  No evidence of vertical transmission of naturally acquired feline immunodeficiency virus infection. , 1992, Veterinary immunology and immunopathology.

[7]  R. Kurth,et al.  Quantitation of a lentivirus in its natural host: simian immunodeficiency virus in African green monkeys , 1992, Journal of virology.

[8]  A. Hasegawa,et al.  Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. , 1992, The Journal of veterinary medical science.

[9]  T. Furuya,et al.  Identification of feline immunodeficiency virus rev gene activity , 1991, Journal of virology.

[10]  J. Albert,et al.  Few infected CD4+ T cells but a high proportion of replication-competent provirus copies in asymptomatic human immunodeficiency virus type 1 infection , 1991, Journal of virology.

[11]  J. Genescà,et al.  Clinical correlation and genetic polymorphism of the human immunodeficiency virus proviral DNA obtained after polymerase chain reaction amplification. , 1990, The Journal of infectious diseases.

[12]  M. Baseler,et al.  Increasing viral burden in CD4+ T cells from patients with human immunodeficiency virus (HIV) infection reflects rapidly progressive immunosuppression and clinical disease. , 1990, Annals of internal medicine.

[13]  S. Dandekar,et al.  Quantitation of human immunodeficiency virus DNA by using the polymerase chain reaction , 1990, Journal of clinical microbiology.

[14]  S. Oka,et al.  Quantitative analysis of human immunodeficiency virus type-1 DNA in asymptomatic carriers using the polymerase chain reaction. , 1990, Biochemical and biophysical research communications.

[15]  N. Pedersen,et al.  Feline leukemia virus infection as a potentiating cofactor for the primary and secondary stages of experimentally induced feline immunodeficiency virus infection , 1990, Journal of virology.

[16]  P. Simmonds,et al.  Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers , 1990, Journal of virology.

[17]  A. Collier,et al.  Plasma viremia in human immunodeficiency virus infection. , 1989, The New England journal of medicine.

[18]  D. Ho,et al.  Quantitation of human immunodeficiency virus type 1 in the blood of infected persons. , 1989, The New England journal of medicine.

[19]  M. Baseler,et al.  Integrated proviral human immunodeficiency virus type 1 is present in CD4+ peripheral blood lymphocytes in healthy seropositive individuals , 1989, Journal of virology.

[20]  S. Crispin,et al.  Clinical and laboratory findings in cats infected with feline immunodeficiency virus , 1989, Veterinary Record.

[21]  N. Pedersen,et al.  Nucleotide sequence and genomic organization of feline immunodeficiency virus. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[22]  C H Fox,et al.  The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4. , 1989, Science.

[23]  H. Lutz,et al.  Prevalence of antibody to feline immunodeficiency virus in some cat populations , 1989, Veterinary Record.

[24]  N. Pedersen,et al.  Epidemiologic and clinical aspects of feline immunodeficiency virus infection in cats from the continental United States and Canada and possible mode of transmission. , 1989, Journal of the American Veterinary Medical Association.

[25]  N. Pedersen,et al.  Feline immunodeficiency virus infection in cats of Japan. , 1989, Journal of the American Veterinary Medical Association.

[26]  W. Corbett,et al.  Seroepidemiologic survey of feline immunodeficiency virus infection in cats of Wake County, North Carolina. , 1989, Journal of the American Veterinary Medical Association.

[27]  N. Pedersen,et al.  Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. , 1988, American journal of veterinary research.

[28]  K. Toriyabe,et al.  日本の飼い猫における猫Tリンパ球指向性レンチウイルス(FTLV)感染症の検出 , 1988 .

[29]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[30]  T. Gruffydd-Jones,et al.  Isolation of a T-lymphotropic lentivirus from a persistently leucopenic domestic cat , 1988, Veterinary Record.

[31]  C Y Ou,et al.  DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. , 1988, Science.

[32]  K. Mullis,et al.  Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection , 1987, Journal of virology.

[33]  N. Pedersen,et al.  Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. , 1987, Science.