Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study.

We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

[1]  D. Richter,et al.  Intrinsic optical signals in respiratory brain stem regions of mice: neurotransmitters, neuromodulators, and metabolic stress. , 2001, Journal of neurophysiology.

[2]  D. Kleinfeld,et al.  Noninvasive detection of changes in membrane potential in cultured neurons by light scattering. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[3]  K. V. Indukaev,et al.  Study of Regular Intracellular and Membrane Processes in Neurons by Laser Interference Microscopy , 2005, Bulletin of Experimental Biology and Medicine.

[4]  A. Grossmann,et al.  DECOMPOSITION OF HARDY FUNCTIONS INTO SQUARE INTEGRABLE WAVELETS OF CONSTANT SHAPE , 1984 .

[5]  H. G. Davies.  STRUCTURE IN NUCLEATED ERYTHROCYTES , 1961, The Journal of biophysical and biochemical cytology.

[6]  M Totzeck,et al.  Interference microscopy of sub-λ structures: A rigorous computation method and measurements , 1997 .

[7]  V. Tychinsky.  On superresolution of phase objects , 1989 .

[8]  Gabriel Popescu,et al.  Erythrocyte structure and dynamics quantified by Hilbert phase microscopy. , 2005, Journal of biomedical optics.

[9]  Erol Başar,et al.  Odor input generates ∼1.5 Hz and ∼3 Hz spectral peaks in the Helix pedal ganglion , 2000, Brain Research.

[10]  R. Dasari,et al.  Diffraction phase microscopy for quantifying cell structure and dynamics. , 2006, Optics letters.

[11]  A. Szűcs,et al.  Periodic and oscillatory firing patterns in identified nerve cells of Lymnaea stagnalis L , 1999, Acta biologica Hungarica.

[12]  A. N. Pavlov,et al.  Unraveling Cell Processes: Interference Imaging Interwoven with Data Analysis , 2006, Journal of biological physics.

[13]  V. P. Tychinskii,et al.  Coherent phase microscopy of intracellular processes , 2001 .

[14]  S. Lux.  Disorders of the red cell membrane , 1995 .

[15]  E Mosekilde,et al.  Interference microscopy under double-wavelet analysis: a new approach to studying cell dynamics. , 2005, Physical review letters.

[16]  E. Cuche,et al.  Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. , 2005, Optics express.

[17]  V. Andreev,et al.  Phase Modulation Microscope MIM-2.1 for Measurements of Surface Microrelief. General Principles of Design and Operation , 2005 .

[18]  S. Boppart,et al.  Functional optical coherence tomography for detecting neural activity through scattering changes. , 2003, Optics letters.

[19]  S. Orlov,et al.  Erythrocyte membrane fluidity and haemoglobin haemoporphyrin conformation: features revealed in patients with heart failure. , 2005, Pathophysiology : the official journal of the International Society for Pathophysiology.

[20]  V. Andreev,et al.  The Problem of Subrayleigh Resolution in Interference Microscopy , 2003 .

[21]  A. Caswell,et al.  Visualization of membrane bound cations by a fluorescent technique. , 1971, Biochemical and biophysical research communications.

[22]  V. Tychinsky.  Wavefront dislocations and registering images inside the Airy disk , 1991 .

[23]  Konstantin V. Indukaev,et al.  The problem of sub-Rayleigh resolution in interference microscopy , 2003, Saratov Fall Meeting.

[24]  V. A. Andreev,et al.  Phase Modulation Microscope MIM-2.1 for Measurements of Surface Microrelief. Results of Measurements , 2005 .

[25]  Hans J. Tiziani,et al.  Phase-singularities in 2D diffraction fields and interference microscopy , 1997 .

[26]  R. Keynes,et al.  Light Scattering and Birefringence Changes during Nerve Activity , 1968, Nature.

[27]  R D Allen,et al.  New observations on cell architecture and dynamics by video-enhanced contrast optical microscopy. , 1985, Annual review of biophysics and biophysical chemistry.

[28]  G. Maksimov,et al.  The Relation of Different-Scale Membrane Processes Under Nitric Oxide Influence , 2005, Journal of biological physics.

[29]  J. D. Angstadt.  Persistent inward currents in cultured Retzius cells of the medicinal leech , 1999, Journal of Comparative Physiology A.

[30]  Gabriel Popescu,et al.  Observation of dynamic subdomains in red blood cells. , 2006, Journal of biomedical optics.