Urinary trypsin inhibitor may have a protective effect on endothelial cells in preeclampsia

Urinary trypsin inhibitor is a glycoprotein which has an inhibitory effect on many enzymes, especially neutrophil elastase. The concentration of urinary trypsin inhibitor in serum (maternal and fetal), urine and amniotic fluid was measured in 20 cases of preeclampsia and compared with levels in normal pregnancy. Urinary trypsin inhibitor levels were significantly increased in the maternal and fetal serum of preeclamptic patients compared to normal pregnancy (p < 0.0001), as well as in urine and amniotic fluid of the same patients. Relative fluorescence release of a calcium chelating agent (fura‐2) from human umbilical vein endothelial cell cultures was significantly increased by preeclamptic serum compared to serum of normal pregnant women (p < 0.03). After incubation of urinary trypsin inhibitor with the cultures, significant decrease of fura‐2 release was observed (p < 0.03).

[1]  Takesada Mori,et al.  Protease inhibitors (gabexate mesylate and ulinastatin) stimulate intracellular chemiluminescence in human neutrophils , 1992, Journal of leukocyte biology.

[2]  A. Bengtsson,et al.  Complement, neutrophil and macrophage activation in women with severe preeclampsia and the syndrome of hemolysis, elevated liver enzymes and low platelet count , 1992, Obstetrics and Gynecology.

[3]  Y. Yamanaka,et al.  Protective effect of ulinastatin against liver injury caused by ischemia-reperfusion in rats. , 1992, Japanese journal of pharmacology.

[4]  K. Ohlsson,et al.  Distribution and elimination of intravenously injected urinary trypsin inhibitor. , 1991, Scandinavian journal of clinical and laboratory investigation.

[5]  M. Balduyck,et al.  [Inter-alpha-trypsin inhibitor and its plasma and urine derivatives]. , 1991, Annales de Biologie Clinique.

[6]  J. Dawes,et al.  Neutrophil activation in pregnancy‐induced hypertension , 1990, British journal of obstetrics and gynaecology.

[7]  T. Musci,et al.  Preeclampsia: an endothelial cell disorder. , 1989, American journal of obstetrics and gynecology.

[8]  K. Ohlsson,et al.  Radioimmunological quantitation of the urinary trypsin inhibitor in normal blood and urine. , 1989, Biological chemistry Hoppe-Seyler.

[9]  I. Morita,et al.  A new in vitro method using fura-2 for the quantification of endothelial cell injury. , 1988, Prostaglandins, Leukotrienes and Essential Fatty Acids.

[10]  J. Harlan.  Neutrophil-mediated vascular injury. , 2009, Acta medica Scandinavica. Supplementum.

[11]  S. Nishibe,et al.  Effect of human urinary trypsin inhibitor on granulocyte elastase activity. , 1987, Research communications in chemical pathology and pharmacology.

[12]  I. Byrjalsen,et al.  Human inter-alpha-trypsin inhibitor and immunologically related inhibitors investigated by quantitative immunoelectrophoresis. II. Pathological conditions. , 1987, Clinica chimica acta; international journal of clinical chemistry.

[13]  M. Balduyck,et al.  Human urinary proteinase inhibitor: inhibitory properties and interaction with bovine trypsin. , 1985, Biological chemistry Hoppe-Seyler.

[14]  H. Faarvang.  The excretion of trypsin inhibitor in urine during normal pregnancy. , 1959, Acta endocrinologica.