Effect of intraperitoneal injection with chlorobenzene on glutathione metabolism in rat liver.

Effect of chlorobenzene administration on glutathione metabolism in rat liver was investigated. rats were intraperitoneally injected with chlorobenzene (2.0 mmol/kg). At 6 hours, hepatic total and oxidized glutathione in the rat with chlorobenzene decreased to 24% and 53% of those in control rats without chlorobenzene, respectively. When 24 hours passed after the injection, the total and oxidized glutathione increased to 188% and 170% of the control, respectively. And simultaneously, the activities of hepatic glutathione synthesis and glutathione r eductase elevated to 193% and 136% of the control, respectively. At 48 hours, these elevation were still found and the liver was enlarged with significant increase in the protein and DNA. The activities of hepatic ƒÁ-glutamyltranspeptidase and glutathione peroxidase were unaltered by the chlorobenzene. Similarly to the single injection, four times intraperitoneal injection with chlorobenzene (2.0 mmol/kg/one shot at 48 hours-intervals) caused the hepatic enlargement and the accumulation of hepatic glutathione. These results indicate that the acceleration of hepatic glutathione synthesis, occurring in response to the transient decrease of glutathione, caused the accumulation of glutathione in liver of rat injected with chlorobenzene.

[1]  W. Hoekstra,et al.  Adaptive changes in hepatic glutathione metabolism in response to excess selenium in rats. , 1983, The Journal of nutrition.

[2]  A. Greenwell,et al.  Relationship of tissue nonprotein/sulfhydryls to the acute toxic effects of 1,2-dibromo-3-chloropropane. , 1982, The Journal of pharmacology and experimental therapeutics.

[3]  Munehiro Yoshida,et al.  Distribution of Selenium in Bovine Milk and Selenium Deficiency in Rats Fed Casein-based Diets, Monitored by Lipid Peroxide Level and Glutathione Peroxidase Activity , 1981 .

[4]  N. Sahyoun,et al.  Tissue glutathione as a cyst(e)ine reservoir during fasting and refeeding of rats. , 1981, The Journal of nutrition.

[5]  E. Bresnick.  Chapter 4 – Induction of the Enzymes of Detoxication , 1980 .

[6]  S. Orrenius,et al.  Metabolic activation and hepatotoxicity. Effect of cysteine, N-acetylcysteine, and methionine on glutathione biosynthesis and bromobenzene toxicity in isolated rat hepatocytes. , 1979, Archives of biochemistry and biophysics.

[7]  H. Mitsuda,et al.  Purification and enzymatic properties of gamma-glutamyltransferase from bovine colostrum. , 1978, Journal of biochemistry.

[8]  M. Uchiyama,et al.  Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. , 1978, Analytical biochemistry.

[9]  E. Kosower,et al.  The glutathione status of cells. , 1978, International review of cytology.

[10]  T. Galeotti,et al.  Different contribution of rat liver microsomal pigments in the formation of superoxide anions and hydrogen peroxide during development. , 1977, Archives of biochemistry and biophysics.

[11]  T. Higashi,et al.  A novel physiological role of liver glutathione as a reservoir of L-cysteine. , 1977, Journal of biochemistry.

[12]  T. Higashi,et al.  Rat liver glutathione: possible role as a reservoir of cysteine. , 1977, The Journal of nutrition.

[13]  H. B. Burch,et al.  Enzymatic assay for glutathione. , 1976, Analytical biochemistry.

[14]  A. Meister,et al.  Glutathione and related gamma-glutamyl compounds: biosynthesis and utilization. , 1976, Annual review of biochemistry.

[15]  S. Thorgeirsson,et al.  Acetaminophen-induced hepatic necrosis. VI. Metabolic disposition of toxic and nontoxic doses of acetaminophen. , 1974, Pharmacology.

[16]  D. Jollow,et al.  Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. , 1974, Pharmacology.

[17]  B B Brodie,et al.  Acetaminophen-induced hepatic necrosis. IV. Protective role of glutathione. , 1973, The Journal of pharmacology and experimental therapeutics.

[18]  A. Y. Lu,et al.  Lipid peroxidation and the degradation of cytochrome P-450 heme. , 1973, Archives of biochemistry and biophysics.

[19]  G. Krishna,et al.  Centrolobular hepatic necrosis related to covalent binding of metabolites of halogenated aromatic hydrocarbons. , 1973, Experimental and molecular pathology.

[20]  S. Aust,et al.  NADPH-dependent lipid peroxidation catalyzed by purified NADPH-cytochrome c reductase from rat liver microsomes , 1972 .

[21]  B B Brodie,et al.  Possible mechanism of liver necrosis caused by aromatic organic compounds. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[22]  C. Little,et al.  Properties and regulation of glutathione peroxidase. , 1970, The Journal of biological chemistry.

[23]  L. F. Chasseaud,et al.  The effect of some carbonyl compounds on rat liver glutathione levels. , 1970, Biochemical pharmacology.

[24]  F. Tietze.  Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. , 1969, Analytical biochemistry.

[25]  M. Gaitonde.  A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. , 1967, The Biochemical journal.

[26]  V. Massey,et al.  On the reaction mechanism of yeast glutathione reductase. , 1965, The Journal of biological chemistry.

[27]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.

[28]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[29]  W. C. Schneider.  Phosphorus compounds in animal tissues; a comparison of methods for the estimation of nucleic acids. , 1946, The Journal of biological chemistry.