Antioxidants from carbon dioxide fixing Chlorella sorokiniana

Chlorella sorokiniana H-84, which has toleranceto high temperatures and high concentrations ofCO2, has been isolated from a hot spring inJapan. Large-scale culturing of C. sorokinianawas carried out in air containing 10% CO2.Analysis of the biomass shows that protein, carbohydrate and lipids comprised 68.5, 11.9 and10.0% of dry matter, respectively. The totalcarotenoids comprised 0.69% dry matter. The luteinand β-carotene contents were 4300 and 600 μgg-1 dry weight, respectively. Theα-tocopherol content was 112 μg g-1 dry weight. These carotenoids and α-tocopherolare known to possess radical scavenging activity.Two fractions with radical scavenging activity wereisolated from the aqueous extract of C.sorokiniana H-84. The extract showed several singlepeaks by reversed phase HPLC analysis and two of themhad molecular weights of 710 and 1286, respectively.

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