Induction and variants of neuronal nitric oxide synthase type I during synaptogenesis

In the adult central nervous system, nitric oxide (NO) is formed from L‐arginine by the so‐called constitutive or type I NO synthase (NOS‐I155). However, expression of NOS‐I155 immunore‐activity and activity was low or not detectable in developing mouse and rat brain. NOS‐I155 was sharply induced coincident with the onset of synapto‐genesis in specific brain regions. This was followed by a second phase in which total NOS‐I155 expression decreased both in specific cell populations and in the total synaptosomal subcellular fraction. Furthermore, two putative variants of NOS‐I were transiently observed: an NOS‐I‐immunoreactive protein with increased electrophoretic mobility (NOS‐I144) and a transient hypersensitivity of NOS‐I155 to the competitive substrate inhibitor Nω‐nitro‐L‐arginine. It is concluded that NOS‐I expression is not constitutive but locally induced. In the central nervous system, this regionally specific, biphasic pattern of postnatal NOS‐I induction is consistent with a role for NO in synap‐togenesis and synaptic plasticity.—Ogilvie, P., Schilling, K., Billingeley, M. L., Schmidt, H. H. H. W. Induction and variants of neuronal nitric oxide synthase type I during synaptogenesis. FASEBJ. 9, 799‐806 (1995)

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