Streak camera crosstalk reduction using a multiple delay optical fiber bundle.

The streak camera is one of the fastest photodetection systems, while its capability of multiplexing is particularly attractive to many applications requiring parallel data acquisition. The degree of multiplexing in a streak camera is limited by the crosstalk between input channels. We developed a technique that introducing a fixed time delay between adjacent fiber channels in a customized two-dimensional to one-dimensional fiber array to significantly reduce crosstalk both at the sample plane and at the input of a streak camera. A prototype system has been developed that supports 100 input channels, and its performance in fluorescence microscopy is demonstrated.

[1]  A. Campillo,et al.  Picosecond streak camera fluorometry - A review , 1983, IEEE Journal of Quantum Electronics.

[2]  Satoshi Hirayama,et al.  A fast deconvolution method to analyze fluorescence decays when the excitation pulse repetition period is less than the decay times , 1988 .

[3]  M. Clampin,et al.  Spatial resolution characteristics of a GaAs‐photocathode RANICON , 1989 .

[4]  Shigeo Minami,et al.  A Fluorescence Lifetime Distribution Measurement System Based on Phase-Resolved Detection Using an Image Dissector Tube , 1989 .

[5]  M Murata,et al.  Development of a streak-camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells. , 1991, Biochemistry.

[6]  T. French,et al.  Two‐photon fluorescence lifetime imaging microscopy of macrophage‐mediated antigen processing , 1997, Journal of microscopy.

[7]  Georges Wagnières,et al.  Instrumentation for real-time fluorescence lifetime imaging in endoscopy , 1999 .

[8]  Brian Herman,et al.  Development of a multiphoton fluorescence lifetime imaging microscopy system using a streak camera , 2003 .

[9]  V. Centonze,et al.  Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera. , 2003, Journal of biomedical optics.

[10]  Klaus Benndorf,et al.  FRET between cardiac Na+ channel subunits measured with a confocal microscope and a streak camera , 2004, Nature Biotechnology.

[11]  Ammasi Periasamy,et al.  Characterization of two‐photon excitation fluorescence lifetime imaging microscopy for protein localization , 2004, Microscopy research and technique.

[12]  Junle Qu,et al.  Temporally and spectrally resolved sampling imaging with a specially designed streak camera. , 2006, Optics letters.

[13]  Yi Fu,et al.  Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence images and time-resolved studies using single-molecule spectroscopy. , 2006, Analytical chemistry.

[14]  A. Jeyasekharan,et al.  A white light confocal microscope for spectrally resolved multidimensional imaging , 2007, Journal of microscopy.

[15]  Qiyin Fang,et al.  Single-shot acquisition of time-resolved fluorescence spectra using a multiple delay optical fiber bundle. , 2008, Optics letters.

[16]  Mauro Nisoli,et al.  New frontiers in attosecond science , 2009 .

[17]  Junle Qu,et al.  High-speed spectrally resolved multifocal multiphoton microscopy , 2010 .

[18]  Qiyin Fang,et al.  Effects of incomplete decay in fluorescence lifetime estimation , 2011, Biomedical optics express.

[19]  R. Day,et al.  Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy , 2011, Nature Protocols.