DNA functionalized gold nanoparticles for bioanalysis.

Gold nanoparticles (Au NPs) have become one of the most interesting sensing materials because of their unique size- and shape-dependent optical properties, high extinction coefficients, and super-quenching capability. Au NPs that are bioconjugated with DNA (DNA-Au NPs) have been demonstrated for selective and sensitive detection of analytes such as mercury(ii) ions, platelet-derived growth factor (PDGF), and adenosine triphosphate (ATP). This review focuses on approaches using DNA-Au NPs for colorimetric, fluorescent, and scattering detection of biopolymers and small solutes. We highlight the important roles that the size and concentration of Au NPs, the length and sequence of DNA, the nature of the capping agents, and the ionic strength and pH of solution play in determining the specificity and sensitivity of the nanosensors for the analytes. The advantages and disadvantages of different detection methods for sensing of interesting analytes using DNA-Au NPs will be discussed.

[1]  J. Storhoff,et al.  A DNA-based method for rationally assembling nanoparticles into macroscopic materials , 1996, Nature.

[2]  Weihong Tan,et al.  Multicolor FRET silica nanoparticles by single wavelength excitation. , 2006, Nano letters.

[3]  P. Upadhyay,et al.  Visual detection of IS6110 of Mycobacterium tuberculosis in sputum samples using a test based on colloidal gold and latex beads. , 2006, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[4]  I. Warner,et al.  Gold nanoparticle sensor for homocysteine thiolactone-induced protein modification. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[5]  Chad A. Mirkin,et al.  DNA-Directed Synthesis of Binary Nanoparticle Network Materials , 1998 .

[6]  A. Lipton,et al.  Platelets as a source of fibroblast growth-promoting activity. , 1974, Experimental cell research.

[7]  Zong-Hong Lin,et al.  Bioconjugated gold nanodots and nanoparticles for protein assays based on photoluminescence quenching. , 2008, Analytical chemistry.

[8]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[9]  H. Mattoussi,et al.  Preparation of quantum dot-biotin conjugates and their use in immunochromatography assays. , 2003, Analytical chemistry.

[10]  Angelika Niemz,et al.  Isothermal DNA amplification coupled with DNA nanosphere-based colorimetric detection. , 2005, Analytical chemistry.

[11]  Yu-Fen Huang,et al.  Growth of various Au–Ag nanocomposites from gold seeds in amino acid solutions , 2006 .

[12]  Sanjay Tyagi,et al.  Molecular Beacons: Probes that Fluoresce upon Hybridization , 1996, Nature Biotechnology.

[13]  M. Famulok,et al.  Oligonucleotide libraries--variatio delectat. , 1998, Current opinion in chemical biology.

[14]  P. Ray.  Diagnostics of single base-mismatch DNA hybridization on gold nanoparticles by using the hyper-Rayleigh scattering technique. , 2006, Angewandte Chemie.

[15]  M. Famulok,et al.  Aptamers as tools in molecular biology and immunology. , 1999, Current topics in microbiology and immunology.

[16]  C. Keating,et al.  Nanoscience enables ultrasensitive detection of Alzheimer's biomarker. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[17]  C. Mirkin,et al.  A gold-nanoparticle-based real-time colorimetric screening method for endonuclease activity and inhibition. , 2007, Angewandte Chemie.

[18]  Jian Wang,et al.  One-step label-free optical genosensing system for sequence-specific DNA related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods. , 2008, Analytical chemistry.

[19]  J. Matthew Mauro,et al.  Long-term multiple color imaging of live cells using quantum dot bioconjugates , 2003, Nature Biotechnology.

[20]  E. Lingen,et al.  Coloured gold alloys , 1999 .

[21]  H. Zou,et al.  Polymer/silica nanocomposites: preparation, characterization, properties, and applications. , 2008, Chemical reviews.

[22]  S. Nie,et al.  Self-assembled nanoparticle probes for recognition and detection of biomolecules. , 2002, Journal of the American Chemical Society.

[23]  Huan-Tsung Chang,et al.  Protein-Conjugated Quantum Dots for Detecting Trypsin and Trypsin Inhibitor Through Fluorescence Resonance Energy Transfer , 2007 .

[24]  Chih-Ching Huang,et al.  Aptamer-functionalized gold nanoparticles for turn-on light switch detection of platelet-derived growth factor. , 2007, Analytical chemistry.

[25]  Michael Vollmer,et al.  Optical properties of metal clusters , 1995 .

[26]  C. Mirkin,et al.  Two-color labeling of oligonucleotide arrays via size-selective scattering of nanoparticle probes. , 2001, Journal of the American Chemical Society.

[27]  X. Liu,et al.  A Gold Nanoparticle‐Based Aptamer Target Binding Readout for ATP Assay , 2007 .

[28]  Yi Lu,et al.  A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles. , 2003, Journal of the American Chemical Society.

[29]  D. Reinhoudt,et al.  Fluorescence quenching of dye molecules near gold nanoparticles: radiative and nonradiative effects. , 2002, Physical review letters.

[30]  Juewen Liu,et al.  Fast colorimetric sensing of adenosine and cocaine based on a general sensor design involving aptamers and nanoparticles. , 2005, Angewandte Chemie.

[31]  S. Katz.  The Reversible Reaction of Sodium Thymonucleate and Mercuric Chloride , 1952 .

[32]  Chih-Ching Huang,et al.  Colorimetric determination of urinary adenosine using aptamer-modified gold nanoparticles. , 2008, Biosensors & bioelectronics.

[33]  C. Niemeyer,et al.  Sensitive detection of proteins using difunctional DNA-gold nanoparticles. , 2005, Small.

[34]  W. Snider,et al.  Developmental expression of the platelet-derived growth factor alpha-receptor gene in mammalian central nervous system. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[35]  M. Chandrasekhar,et al.  Agarose-stabilized gold nanoparticles for surface-enhanced Raman spectroscopic detection of DNA nucleosides , 2006 .

[36]  J. Aveyard,et al.  One molecule per particle method for functionalising nanoparticles. , 2004, Chemical communications.

[37]  Chaoqing Dong,et al.  Single‐Molecule Technology for Rapid Detection of DNA Hybridization Based on Resonance Light Scattering of Gold Nanoparticles , 2007, Chembiochem : a European journal of chemical biology.

[38]  U. Hellman,et al.  A major part of platelet-derived growth factor purified from human platelets is a heterodimer of one A and one B chain. , 1988, The Journal of biological chemistry.

[39]  Huixiang Li,et al.  Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[40]  Chih-Ching Huang,et al.  Synthesis of fluorescent carbohydrate-protected Au nanodots for detection of Concanavalin A and Escherichia coli. , 2009, Analytical chemistry.

[41]  Yingfu Li,et al.  Simple and Rapid Colorimetric Biosensors Based on DNA Aptamer and Noncrosslinking Gold Nanoparticle Aggregation , 2007, Chembiochem : a European journal of chemical biology.

[42]  Chih-Ching Huang,et al.  Selective gold-nanoparticle-based "turn-on" fluorescent sensors for detection of mercury(II) in aqueous solution. , 2006, Analytical chemistry.

[43]  A. Libchaber,et al.  Single-mismatch detection using gold-quenched fluorescent oligonucleotides , 2001, Nature Biotechnology.

[44]  A. Heeger,et al.  Beyond superquenching: Hyper-efficient energy transfer from conjugated polymers to gold nanoparticles , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[45]  Zusing Yang,et al.  Anisotropic syntheses of boat-shaped core–shell Au–Ag nanocrystals and nanowires , 2006 .

[46]  H. Kim,et al.  Study of electrolyte induced aggregation of gold nanoparticles capped by amino acids. , 2006, Journal of colloid and interface science.

[47]  Soumen Basu,et al.  Glutathione-induced aggregation of gold nanoparticles: electromagnetic interactions in a closely packed assembly. , 2007, Journal of nanoscience and nanotechnology.

[48]  Chih-Ching Huang,et al.  Detection of mercury(II) based on Hg2+ -DNA complexes inducing the aggregation of gold nanoparticles. , 2008, Chemical communications.

[49]  Itamar Willner,et al.  Optical analysis of Hg2+ ions by oligonucleotide-gold-nanoparticle hybrids and DNA-based machines. , 2008, Angewandte Chemie.

[50]  C. Mirkin,et al.  Scanometric DNA array detection with nanoparticle probes. , 2000, Science.

[51]  Mizuo Maeda,et al.  Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization. , 2003, Journal of the American Chemical Society.

[52]  John A Rogers,et al.  Nanostructured plasmonic sensors. , 2008, Chemical reviews.

[53]  C. Robic,et al.  Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. , 2008, Chemical reviews.

[54]  Juewen Liu,et al.  Accelerated color change of gold nanoparticles assembled by DNAzymes for simple and fast colorimetric Pb2+ detection. , 2004, Journal of the American Chemical Society.

[55]  Chad A Mirkin,et al.  Homogeneous detection of nucleic acids based upon the light scattering properties of silver-coated nanoparticle probes. , 2007, Analytical chemistry.

[56]  Tarasankar Pal,et al.  Interparticle coupling effect on the surface plasmon resonance of gold nanoparticles: from theory to applications. , 2007, Chemical reviews.

[57]  Han Nim Choi,et al.  Nafion-stabilized magnetic nanoparticles (Fe3O4) for [Ru(bpy)3]2+(bpy = bipyridine) electrogenerated chemiluminescence sensor. , 2005, Chemical communications.

[58]  Yu-Fen Huang,et al.  Synthesis and characterization of Au core-Au-Ag shell nanoparticles from gold seeds: impacts of glycine concentration and pH. , 2006, Journal of colloid and interface science.

[59]  Vinay Gupta,et al.  Reversible aggregation of gold nanoparticles induced by pH dependent conformational transitions of a self-assembled polypeptide. , 2007, Journal of colloid and interface science.

[60]  Zong-Hong Lin,et al.  Determining enediol compounds in tea using surface-assisted laser desorption/ionization mass spectrometry with titanium dioxide nanoparticle matrices. , 2007, Rapid communications in mass spectrometry : RCM.

[61]  D. Astruc,et al.  Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. , 2004, Chemical reviews.

[62]  Surat Hotchandani,et al.  Electrochemical modulation of fluorophore emission on a nanostructured gold film. , 2002, Angewandte Chemie.

[63]  Xiaofang Hu,et al.  Unmodified gold nanoparticles as a colorimetric probe for potassium DNA aptamers. , 2006, Chemical communications.

[64]  Zusing Yang,et al.  Synthesis of highly fluorescent gold nanoparticles for sensing mercury(II). , 2007, Angewandte Chemie.

[65]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[66]  Chad A Mirkin,et al.  Bio-bar-code-based DNA detection with PCR-like sensitivity. , 2004, Journal of the American Chemical Society.

[67]  Chih-Ching Huang,et al.  Control over surface DNA density on gold nanoparticles allows selective and sensitive detection of mercury(II). , 2008, Langmuir : the ACS journal of surfaces and colloids.

[68]  Yi Xiao,et al.  Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. , 2004, Journal of the American Chemical Society.

[69]  C. Mirkin,et al.  A gold nanoparticle/latex microsphere-based colorimetric oligonucleotide detection method , 2000 .

[70]  E. Wang,et al.  Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes. , 2007, Chemical communications.

[71]  Huixiang Li,et al.  Label-free colorimetric detection of specific sequences in genomic DNA amplified by the polymerase chain reaction. , 2004, Journal of the American Chemical Society.

[72]  Ronghua Yang,et al.  Gold nanoparticle-based colorimetric and "turn-on" fluorescent probe for mercury(II) ions in aqueous solution. , 2008, Analytical chemistry.

[73]  Chojiro Kojima,et al.  15N-15N J-coupling across Hg(II): direct observation of Hg(II)-mediated T-T base pairs in a DNA duplex. , 2007, Journal of the American Chemical Society.

[74]  Chih-Ching Huang,et al.  Parameters for selective colorimetric sensing of mercury(II) in aqueous solutions using mercaptopropionic acid-modified gold nanoparticles. , 2007, Chemical communications.

[75]  R. Ross,et al.  A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[76]  Weihong Tan,et al.  Aptamer-modified gold nanoparticles for colorimetric determination of platelet-derived growth factors and their receptors. , 2005, Analytical chemistry.

[77]  R. Ross,et al.  Proteoglycan distribution in lesions of atherosclerosis depends on lesion severity, structural characteristics, and the proximity of platelet-derived growth factor and transforming growth factor-beta. , 1998, The American journal of pathology.

[78]  Robert Wilson.  The use of gold nanoparticles in diagnostics and detection. , 2008, Chemical Society reviews.

[79]  Yi Lu,et al.  Label‐Free Colorimetric Detection of Lead Ions with a Nanomolar Detection Limit and Tunable Dynamic Range by using Gold Nanoparticles and DNAzyme , 2008 .

[80]  Huan‐Tsung Chang,et al.  Synthesis of dumbbell-shaped Au-Ag core-shell nanorods by seed-mediated growth under alkaline conditions. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[81]  Xiaogang Liu,et al.  One-step, room temperature, colorimetric detection of mercury (Hg2+) using DNA/nanoparticle conjugates. , 2008, Journal of the American Chemical Society.

[82]  T. Yamane,et al.  On the Complexing of Desoxyribonucleic Acid (DNA) by Mercuric Ion1 , 1961 .

[83]  Igor L. Medintz,et al.  Multiplexed toxin analysis using four colors of quantum dot fluororeagents. , 2004, Analytical chemistry.

[84]  K. Naka,et al.  pH Responsive Aggregation of Imidazolium Cations-Modified Gold Nanoparticles with Poly(acrylic acid) in Aqueous Solution , 2007 .

[85]  C. Mirkin,et al.  Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins , 2003, Science.

[86]  Jian-hui Jiang,et al.  A colorimetric method for point mutation detection using high-fidelity DNA ligase , 2005, Nucleic acids research.

[87]  Takashi Fujimoto,et al.  MercuryII-mediated formation of thymine-HgII-thymine base pairs in DNA duplexes. , 2006, Journal of the American Chemical Society.

[88]  Zong-Hong Lin,et al.  Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering , 2009 .

[89]  C. Mirkin,et al.  Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection. , 2002, Science.