Synthesis and In Vitro Antitumor Activities of Lupanol Gallate

Under the presence of N,N'-dicyclohexylcarbodiimide (DDC) as dehydrating agent and 4-dimethylaminopyridine (DMAP) as catalyst, lupanol was refluxed with excessive gallic acid for 10 h in dichloromethane to give lupanol gallate as a new compound at 69.6% yield. The latter was explored for in vitro antitumor activities against A549, LAC and HepG2 cell lines. The results showed that lupanol and cisplatin as positive control compounds had no inhibitory abilities against the above three tested tumor cell lines. The IC50 values of lupanol gallate against A549, LAC and HepG2 cells were 51.71 μM, 62.16 μM and 64.34 μM respectively. The antitumor activities of lupanol gallate against A549 and LAC cells were inferior to those of adriamycin, however its antitumor activity against HepG2 cell exceeded that of adriamycin.