Inertial microfluidics combined with selective cell lysis for high throughput separation of nucleated cells from whole blood

The ability to rapidly analyze and extract information from peripheral blood cells has the potential of providing a wealth of new information about immune function and general health of the patient ...

[1]  Yi-Kuen Lee,et al.  Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers. , 2011, Angewandte Chemie.

[2]  Jongyoon Han,et al.  Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics , 2015, Nature Protocols.

[3]  A. Raj,et al.  Exploiting osmosis for blood cell sorting , 2011, Biomedical microdevices.

[4]  Mehmet Toner,et al.  Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis. , 2006, Analytical chemistry.

[5]  S. Digumarthy,et al.  Isolation of rare circulating tumour cells in cancer patients by microchip technology , 2007, Nature.

[6]  Elisabeth Smela,et al.  High-throughput particle separation and concentration using spiral inertial filtration. , 2014, Biomicrofluidics.

[7]  A. Ladd,et al.  Inertial migration of neutrally buoyant particles in a square duct: An investigation of multiple equilibrium positions , 2006 .

[8]  Yu Sun,et al.  Microfluidic approaches for cancer cell detection, characterization, and separation. , 2012, Lab on a chip.

[9]  Mehmet Toner,et al.  A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects. , 2007, Lab on a chip.

[10]  Eugene J. Lim,et al.  Microfluidic, marker-free isolation of circulating tumor cells from blood samples , 2014, Nature Protocols.

[11]  A. Bhagat,et al.  Inertial microfluidics for continuous particle separation in spiral microchannels. , 2009, Lab on a chip.

[12]  A. Bhagat,et al.  Separation of leukocytes from blood using spiral channel with trapezoid cross-section. , 2012, Analytical chemistry.

[13]  Kun Huang,et al.  Injury, Large-Scale Collaborative Research Program , 2015 .

[14]  S. Groshen,et al.  Portable Filter-Based Microdevice for Detection and Characterization of Circulating Tumor Cells , 2010, Clinical Cancer Research.

[15]  Elisabeth Guazzelli,et al.  Lateral force on a rigid sphere in large-inertia laminar pipe flow , 2009, Journal of Fluid Mechanics.

[16]  Kyung-A Hyun,et al.  Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation. , 2013, Biomicrofluidics.

[17]  Markus Beck,et al.  Filter Characteristics Influencing Circulating Tumor Cell Enrichment from Whole Blood , 2013, PloS one.

[18]  T. Franke,et al.  Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift. , 2013, Biomicrofluidics.

[19]  Peter C. Y. Chen,et al.  Spiral microchannel with rectangular and trapezoidal cross-sections for size based particle separation , 2013, Scientific Reports.

[20]  Peter C. Y. Chen,et al.  Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells. , 2014, Lab on a chip.

[21]  V. Vandelinder,et al.  Perfusion in microfluidic cross-flow: separation of white blood cells from whole blood and exchange of medium in a continuous flow. , 2007, Analytical chemistry.

[22]  H. Brismar,et al.  Microfluidic-based isolation of bacteria from whole blood for sepsis diagnostics , 2014, Biotechnology Letters.

[23]  A. Bhagat,et al.  Continuous particle separation in spiral microchannels using Dean flows and differential migration. , 2008, Lab on a chip.

[24]  R. Tompkins,et al.  Continuous inertial focusing, ordering, and separation of particles in microchannels , 2007, Proceedings of the National Academy of Sciences.

[25]  L Vettore,et al.  A new density gradient system for the separation of human red blood cells , 1980, American journal of hematology.

[26]  Paul I. Okagbare,et al.  Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor. , 2008, Journal of the American Chemical Society.

[27]  C. Lim,et al.  Isolation and retrieval of circulating tumor cells using centrifugal forces , 2013, Scientific Reports.

[28]  Weihong Tan,et al.  Aptamer-based microfluidic device for enrichment, sorting, and detection of multiple cancer cells. , 2009, Analytical chemistry.

[29]  Han Wei Hou,et al.  Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation. , 2011, Lab on a chip.

[30]  Mehmet Toner,et al.  Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patients. , 2008, Clinical chemistry.

[31]  Mehmet Toner,et al.  Continuous flow microfluidic device for rapid erythrocyte lysis. , 2004, Analytical chemistry.

[32]  D. Drew.  The lift force on a small sphere in the presence of a wall , 1988 .

[33]  L. Talbot,et al.  Flow in Curved Pipes , 1983 .

[34]  Rohit Karnik,et al.  Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns , 2013, Scientific Reports.

[35]  Sungyoung Choi,et al.  Inertial modulation of hydrophoretic cell sorting and focusing , 2014 .

[36]  M. Toner,et al.  Differential inertial focusing of particles in curved low-aspect-ratio microchannels , 2009, New journal of physics.

[37]  Tommy Haraldsson,et al.  Inertial microfluidics in parallel channels for high-throughput applications. , 2012, Lab on a chip.

[38]  Sung Yang,et al.  A microfluidic device for continuous white blood cell separation and lysis from whole blood. , 2010, Artificial organs.

[39]  Donald Wlodkowic,et al.  Immunology on chip: promises and opportunities. , 2014, Biotechnology advances.

[40]  R. Tompkins,et al.  Equilibrium separation and filtration of particles using differential inertial focusing. , 2008, Analytical chemistry.

[41]  Maciej Zborowski,et al.  Enrichment of rare cancer cells through depletion of normal cells using density and flow-through, immunomagnetic cell separation. , 2004, Experimental hematology.

[42]  Maciej Zborowski,et al.  Magnetic cell separation: characterization of magnetophoretic mobility. , 2003, Analytical chemistry.

[43]  R. Hoffman,et al.  In Vitro Culture and Characterization of Human Lung Cancer Circulating Tumor Cells Isolated by Size Exclusion from an Orthotopic Nude-Mouse Model Expressing Fluorescent Protein , 2014, Journal of Fluorescence.

[44]  P. Black,et al.  Highly selective biomechanical separation of cancer cells from leukocytes using microfluidic ratchets and hydrodynamic concentrator. , 2013, Biomicrofluidics.

[45]  Ian Papautsky,et al.  Continuous separation of blood cells in spiral microfluidic devices. , 2013, Biomicrofluidics.

[46]  Xingyu Jiang,et al.  Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels. , 2013, Biomicrofluidics.

[47]  G. Hodge,et al.  Changes in leukocyte subsets: clinical implications for children with chronic renal failure , 2005, Pediatric Nephrology.

[48]  Tae Hyun Kim,et al.  Cascaded spiral microfluidic device for deterministic and high purity continuous separation of circulating tumor cells. , 2014, Biomicrofluidics.

[49]  Fei Huang,et al.  Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure. , 2013, Biomicrofluidics.

[50]  H. Chang,et al.  Cell retention culture with an internal filter module: Continuous ethanol fermentation , 1993, Biotechnology and bioengineering.

[51]  K. Madyastha,et al.  An improved method for rapid layering of Ficoll-Hypaque double density gradients suitable for granulocyte separation. , 1982, Journal of immunological methods.

[52]  F F Becker,et al.  Differential analysis of human leukocytes by dielectrophoretic field-flow-fractionation. , 2000, Biophysical journal.

[53]  Elisabeth Guazzelli,et al.  Lateral Forces on a Sphere , 2004 .

[54]  J. Harris,et al.  Relationship of leukocyte numbers, immunoregulatory cell function, and phytohemagglutinin responsiveness in cancer patients. , 1981, Journal of the National Cancer Institute.

[55]  S. Matsusaka,et al.  A novel detection strategy for living circulating tumor cells using 5-aminolevulinic acid. , 2014, Cancer letters.

[56]  Ciprian Iliescu,et al.  Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives. , 2013, Biomicrofluidics.

[57]  Jesper Gantelius,et al.  Dean flow-coupled inertial focusing in curved channels. , 2014, Biomicrofluidics.

[58]  Evgeny S. Asmolov,et al.  The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number , 1999, Journal of Fluid Mechanics.