Morphometric assessments for the healthy rat hepatocytes

Summary The purpose of this study is to perform an evaluati on of representative size and shape parameters that characterise the hepatocytes in nor mal rat livers. A set of thirty digital images of rat hepatocytes in normal rat livers, randomly s elected from a database, were evaluated. Image processing and analysis of digital images wer e performed with ImageJ and MRI Cell Image Analyzer (MRI-CIA) softwares. We found a set of representative size and shape parameters for quantification of hepatocytes in hea lthy rats. The central tendency and dispersion measure of the parameters were expressed by the mean value and standard deviation. The computerized geometric morphometric analysis was in agreement with the ultrastructural observations. Geometrical morphometric analysis of the normal rat livers structure is an efficient noninvasive prediction to ol for analysis of rat histological images.

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