Effect of low temperature on stability of theta-type plasmids in Carnobacterium maltaromaticum.

The heterologous production of useful peptides such as bacteriocins by lactic acid bacteria (LAB) has been studied for use in the biopreservation of foods. Recombinant plasmids can suffer drawbacks such as segregational instability affecting the production of these peptides in certain environments such as absence of selective pressure or low temperature. The link between growth temperature characteristics of parental strains and stability of theta-type plasmids at a low temperature was investigated. The growth of four parental strains at 4 degrees C and stability of five derivative theta-type plasmids transformed into Carnobacterium maltaromaticum UAL26 at 25 and 4 degrees C were determined. Two plasmids (pCD11 and pCaT) derived from psychrotrophic LAB and plasmid, pHW800, from Enterococcus faecium 226 with unknown growth temperature characteristics, had excellent stability when strains were grown at 4 degrees C. Plasmids (pTRKH2 and pUCB820) derived from LAB that did not grow at refrigeration temperatures were not stable at 4 degrees C. When a DNA fragment from pCD11 containing 22-bp repeats, a putative replication initiation site, and the gene for the RepA protein was inserted into pTRKH2, the resulting derivative plasmid was 100% stable at 4 degrees C.

[1]  H. Morita,et al.  Behavior of Psychrotrophic Lactic Acid Bacteria Isolated from Spoiling Cooked Meat Products , 2003, Applied and Environmental Microbiology.

[2]  J. M. Rodríguez,et al.  Heterologous production of bacteriocins by lactic acid bacteria. , 2003, International journal of food microbiology.

[3]  J. Seegers.  Lactobacilli as live vaccine delivery vectors: progress and prospects. , 2002, Trends in biotechnology.

[4]  P. Renault,et al.  Food-Grade Delivery System for Controlled Gene Expression in Lactococcus lactis , 2002, Applied and Environmental Microbiology.

[5]  M. Mataragas,et al.  Microbial interaction in cooked cured meat products under vacuum or modified atmosphere at 4°C , 2002 .

[6]  M. Delley,et al.  Lactobacillus bulgaricus Proteinase Expressed in Lactococcus lactis Is a Powerful Carrier for Cell Wall-Associated and Secreted Bovine β-Lactoglobulin Fusion Proteins , 2002, Applied and Environmental Microbiology.

[7]  K. Björkroth,et al.  Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity , 2002, Applied and Environmental Microbiology.

[8]  Y. Le Loir,et al.  Production and Targeting of the Brucella abortus Antigen L7/L12 in Lactococcus lactis: a First Step towards Food-Grade Live Vaccines against Brucellosis , 2002, Applied and Environmental Microbiology.

[9]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[10]  N. Dunn,et al.  Nucleotide sequence and thermostability of pND324, a 3.6-kb plasmid from Lactococcus lactis , 1999, Applied Microbiology and Biotechnology.

[11]  T. Klaenhammer,et al.  Colicin V can be produced by lactic acid bacteria , 1999, Letters in applied microbiology.

[12]  Y. Le Loir,et al.  Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system. , 1999, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[13]  J. Vederas,et al.  Genetic Characterization and Heterologous Expression of Brochocin-C, an Antibotulinal, Two-Peptide Bacteriocin Produced by Brochothrix campestris ATCC 43754 , 1998, Applied and Environmental Microbiology.

[14]  V. Eijsink,et al.  A system for heterologous expression of bacteriocins in Lactobacillus sake. , 1998, FEMS microbiology letters.

[15]  Y. Auffray,et al.  A new theta-type thermosensitive replicon from Lactococcus lactis as an integration vector for Enterococcus faecalis. , 1998, FEMS microbiology letters.

[16]  M. Gasson,et al.  Production of Pediocin PA-1 by Lactococcus lactis Using the Lactococcin A Secretory Apparatus , 1998, Applied and Environmental Microbiology.

[17]  H. Israelsen,et al.  Heterologous gene expression of bovine plasmin in Lactococcus lactis , 1997, Applied Microbiology and Biotechnology.

[18]  Y. Auffray,et al.  Molecular analysis of the replication region of the theta-replicating plasmid pUCL287 from Tetragenococcus (Pediococcus) halophilus ATCC33315 , 1997, Molecular and General Genetics MGG.

[19]  E. Borch,et al.  Bacterial spoilage of meat and cured meat products. , 1996, International journal of food microbiology.

[20]  Jørgen Leisner,et al.  Control of beef spoilage by a sulfide-producing Lactobacillus sake strain with bacteriocinogenic Leuconostoc gelidum UAL187 during anaerobic storage at 2 degrees C , 1996, Applied and environmental microbiology.

[21]  W. Sandine,et al.  Characterization and sequence analysis of a stable cryptic plasmid from Enterococcus faecium 226 and development of a stable cloning vector , 1996, Applied and environmental microbiology.

[22]  T. Abee,et al.  Bacteriocins: modes of action and potentials in food preservation and control of food poisoning. , 1995, International journal of food microbiology.

[23]  M. Stiles,et al.  from Leuconostoc gelidum . the production of the bacteriocin leucocin A Molecular characterization of genes involved in , 1995 .

[24]  J. Vederas,et al.  A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens , 1995, Journal of bacteriology.

[25]  A. Benachour,et al.  pUCL287 plasmid from Tetragenococcus halophila (Pediococcus halophilus) ATCC 33315 represents a new theta-type replicon family of lactic acid bacteria. , 1995, FEMS microbiology letters.

[26]  T. Klaenhammer,et al.  High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. , 1993, Gene.

[27]  S. Bron,et al.  The Mode of Replication Is a Major Factor in Segregational Plasmid Instability in Lactococcus lactis , 1993, Applied and environmental microbiology.

[28]  M. Stiles,et al.  Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus plantarum caTC2R. , 1992, Plasmid.

[29]  J. S. Horng,et al.  Replication and temperature-sensitive maintenance functions of lactose plasmid pSK11L from Lactococcus lactis subsp. cremoris , 1991, Journal of bacteriology.

[30]  D. Collins-Thompson,et al.  Characterization of chloramphenicol resistance inLactobacillus plantarum caTC2 , 1989, Current Microbiology.

[31]  G. Dunny,et al.  Characterization of Three Plasmid Deoxyribonucleic Acid Molecules in a Strain of Streptococcus faecalis: Identification of a Plasmid Determining Erythromycin Resistance , 1974, Journal of bacteriology.

[32]  M. Stiles,et al.  Characterization of the theta-type plasmid pCD3.4 from Carnobacterium divergens, and modulation of its host range by RepA mutation. , 2006, Microbiology.

[33]  M. Mataragas,et al.  Microbial interaction in cooked cured meat products under vacuum or modified atmosphere at 4 degrees C. , 2002, Journal of applied microbiology.