The In Vivo Kinetics of RNA Polymerase II Elongation during Co-Transcriptional Splicing

Kinetic analysis shows that RNA polymerase elongation kinetics are not modulated by co-transcriptional splicing and that post-transcriptional splicing can proceed at the site of transcription without the presence of the polymerase.

[1]  A. Kornblihtt,et al.  The carboxy terminal domain of RNA polymerase II and alternative splicing. , 2010, Trends in biochemical sciences.

[2]  X. Darzacq,et al.  Splicing-independent recruitment of U1 snRNP to a transcription unit in living cells , 2010, Journal of Cell Science.

[3]  Y. Shav-Tal,et al.  Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells , 2010, Nature Cell Biology.

[4]  R. Singer,et al.  The life of an mRNA in space and time , 2010, Journal of Cell Science.

[5]  D. Komura,et al.  A wave of nascent transcription on activated human genes , 2009, Proceedings of the National Academy of Sciences.

[6]  D. Bentley,et al.  "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions. , 2009, Molecular cell.

[7]  R. Padgett,et al.  Rates of in situ transcription and splicing in large human genes , 2009, Nature Structural &Molecular Biology.

[8]  M. Moore,et al.  Meayamycin inhibits pre–messenger RNA splicing and exhibits picomolar activity against multidrug-resistant cells , 2009, Molecular Cancer Therapeutics.

[9]  M. Carmo-Fonseca,et al.  The spliceosome: a self-organized macromolecular machine in the nucleus? , 2009, Trends in cell biology.

[10]  I. Poser,et al.  SR protein family members display diverse activities in the formation of nascent and mature mRNPs in vivo. , 2009, Molecular cell.

[11]  Melissa J. Moore,et al.  Pre-mRNA Processing Reaches Back toTranscription and Ahead to Translation , 2009, Cell.

[12]  Xiang-Dong Fu,et al.  The splicing factor SC35 has an active role in transcriptional elongation , 2008, Nature Structural &Molecular Biology.

[13]  M. Carmo-Fonseca,et al.  The CTD role in cotranscriptional RNA processing and surveillance , 2008, FEBS letters.

[14]  Xiang-Dong Fu,et al.  Functional integration of transcriptional and RNA processing machineries. , 2008, Current opinion in cell biology.

[15]  R. Reed,et al.  Splicing promotes rapid and efficient mRNA export in mammalian cells , 2008, Proceedings of the National Academy of Sciences.

[16]  J. Kjems,et al.  A 5' splice site enhances the recruitment of basal transcription initiation factors in vivo. , 2008, Molecules and Cells.

[17]  S. Scherer A short guide to the human genome , 2008 .

[18]  D. Bentley,et al.  RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes , 2008, Nature Structural &Molecular Biology.

[19]  A. Kornblihtt,et al.  The transcriptional cycle of HIV-1 in real-time and live cells. , 2007, The Journal of cell biology.

[20]  J. Swedlow,et al.  RNA polymerase II transcription in living color , 2007, Nature Structural &Molecular Biology.

[21]  X. Darzacq,et al.  In vivo dynamics of RNA polymerase II transcription , 2007, Nature Structural &Molecular Biology.

[22]  M. Hagiwara,et al.  Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA , 2007, Nature Chemical Biology.

[23]  A. Krainer,et al.  SR proteins function in coupling RNAP II transcription to pre-mRNA splicing. , 2007, Molecular cell.

[24]  P. Silver,et al.  Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. , 2006, Molecular cell.

[25]  K. Neugebauer,et al.  Cotranscriptional coupling of splicing factor recruitment and precursor messenger RNA splicing in mammalian cells , 2006, Nature Structural &Molecular Biology.

[26]  Robert H Singer,et al.  Gene expression within a dynamic nuclear landscape , 2006, The EMBO journal.

[27]  Clifford A. Meyer,et al.  Genomic localization of RNA binding proteins reveals links between pre-mRNA processing and transcription. , 2006, Genome research.

[28]  X. Darzacq,et al.  Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells , 2006, The Journal of cell biology.

[29]  N. Proudfoot,et al.  Exon tethering in transcription by RNA polymerase II. , 2006, Molecular cell.

[30]  S. Lacadie,et al.  Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast. , 2005, Molecular cell.

[31]  Robert H Singer,et al.  Dynamics of transcription and mRNA export. , 2005, Current opinion in cell biology.

[32]  D. Bentley,et al.  Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors. , 2005, Current opinion in cell biology.

[33]  A. Kornblihtt,et al.  Multiple links between transcription and splicing. , 2004, RNA.

[34]  Robert H Singer,et al.  Materials and Methods Som Text Figs. S1 to S8 References and Notes Dynamics of Single Mrnps in Nuclei of Living Cells , 2022 .

[35]  S. Kameoka,et al.  p54nrb associates with the 5′ splice site within large transcription/splicing complexes , 2004, The EMBO journal.

[36]  Thomas Ried,et al.  From Silencing to Gene Expression Real-Time Analysis in Single Cells , 2004, Cell.

[37]  A. Kornblihtt,et al.  A slow RNA polymerase II affects alternative splicing in vivo. , 2003, Molecular cell.

[38]  H. Le Hir,et al.  How introns influence and enhance eukaryotic gene expression. , 2003, Trends in biochemical sciences.

[39]  Y. Shav-Tal,et al.  PSF and p54nrb/NonO – multi‐functional nuclear proteins , 2002, FEBS letters.

[40]  Hiroshi Kimura,et al.  U1 snRNA associates with TFIIH and regulates transcriptional initiation , 2002, Nature Structural Biology.

[41]  A. Furger,et al.  Promoter proximal splice sites enhance transcription. , 2002, Genes & development.

[42]  K. Neugebauer,et al.  On the importance of being co-transcriptional , 2002, Journal of Cell Science.

[43]  D. Weil,et al.  In Vivo Kinetics of mRNA Splicing and Transport in Mammalian Cells , 2002, Molecular and Cellular Biology.

[44]  B. Blencowe,et al.  Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD. , 2002, RNA.

[45]  T. Maniatis,et al.  An extensive network of coupling among gene expression machines , 2002, Nature.

[46]  Qiang Zhou,et al.  Stimulatory effect of splicing factors on transcriptional elongation , 2001, Nature.

[47]  International Human Genome Sequencing Consortium Initial sequencing and analysis of the human genome , 2001, Nature.

[48]  D. Spector,et al.  Visualization of gene activity in living cells , 2000, Nature Cell Biology.

[49]  J. Lewis,et al.  Like attracts like: getting RNA processing together in the nucleus. , 2000, Science.

[50]  T. Misteli,et al.  High mobility of proteins in the mammalian cell nucleus , 2000, Nature.

[51]  R. Singer,et al.  Sensitive and high-resolution detection of RNA in situ. , 2000, Methods in enzymology.

[52]  Tin Wee Tan,et al.  ExInt: an Exon/Intron database , 2000, Nucleic Acids Res..

[53]  R. Reed,et al.  Splicing is required for rapid and efficient mRNA export in metazoans. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[54]  G. Sudlow,et al.  Large-Scale Chromatin Unfolding and Remodeling Induced by VP16 Acidic Activation Domain , 1999, The Journal of cell biology.

[55]  T. Misteli,et al.  RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. , 1999, Molecular cell.

[56]  R. Singer,et al.  Localization of ASH1 mRNA particles in living yeast. , 1998, Molecular cell.

[57]  Tom Misteli,et al.  The dynamics of a pre-mRNA splicing factor in living cells , 1997, Nature.

[58]  M. Roth,et al.  Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. , 1997, Genes & development.

[59]  D. Bregman,et al.  Splicing Factors Associate with Hyperphosphorylated RNA Polymerase II in the Absence of Pre-mRNA , 1997, The Journal of cell biology.

[60]  O. Bensaude,et al.  The nuclear matrix protein p255 is a highly phosphorylated form of RNA polymerase II largest subunit which associates with spliceosomes. , 1996, Nucleic acids research.

[61]  P. Sharp,et al.  A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[62]  R. Palmiter,et al.  Rat growth hormone gene introns stimulate nucleosome alignment in vitro and in transgenic mice. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[63]  H. Klamut,et al.  The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced , 1995, Nature Genetics.

[64]  Michael R. Green,et al.  Localization of pre-mRNA splicing in mammalian nuclei , 1994, Nature.

[65]  L. Chasin,et al.  Order of intron removal during splicing of endogenous adenine phosphoribosyltransferase and dihydrofolate reductase pre-mRNA , 1993, Molecular and cellular biology.

[66]  D. Spector,et al.  Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors. , 1991, Genes & development.

[67]  Y. Osheim,et al.  Splice site selection, rate of splicing, and alternative splicing on nascent transcripts. , 1988, Genes & development.

[68]  S. Weissman,et al.  Accurate in vitro splicing of human β-globin RNA , 1982 .

[69]  S. Weissman,et al.  Accurate in vitro splicing of human beta-globin RNA. , 1982, Nucleic acids research.

[70]  P. Leder,et al.  SV40 recombinants carrying rabbit β-globin gene coding sequences , 1979, Cell.

[71]  P. Leder,et al.  SV40 recombinants carrying rabbit beta-globin gene coding sequences. , 1979, Cell.