论文信息 - Label free detection of quadruplex formation of peroxidase-like DNAzyme by tapered fiber optic biosensor

Label free detection of quadruplex formation of peroxidase-like DNAzyme by tapered fiber optic biosensor

We investigated the possibility the active DNAzyme formation of particular Guanine-rich oligonucleotide with nonadiabatic tapered optical fiber (NATOF) sensor. These kinds of oligonucleotides can form stable four-stranded structure called G-quadruplex in the presence of particular metal ions. Structural change of immobilized oligonucleotide was detected by monitoring of transmission spectrum of the sensor. The formation of active DNAzyme formed was confirmed by verifying enzyme activity. Result shows that the NATOF sensor has capability to detect intramolecular structural changes and using this sequence as biorecognition molecule for fabricating fiber optic biosensor.

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