A Novel Disposable Biosensor Based on SiNWs/AuNPs Modified-Screen Printed Electrode for Dengue Virus DNA Oligomer Detection

In this paper, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. First, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-aminopropyltriethoxysilane (0.5%) onto bare SPGE. Second, the AuNPs decoration on SiNWs-SPGE surface was functionalized using dithiopropionic acid through a self-assembly monolayer technique. The electrochemical response of methylene blue (MB) as a redox indicator toward synthetic DNA oligomer after hybridization on SiNWs/AuNPs-SPGE was recorded by cyclic voltammetry and differential pulse voltammetry techniques. The results demonstrated that the reduction peak current of MB was significantly decreased after DNA hybridization process. In addition, the developed biosensor showed a good storage stability and could achieve a linear range of 1 × 10-11 - 1 × 10-7 M (R = 0.98) with the detection limit of 1.63 × 10-12 M.

[1]  Minho Lee,et al.  The label free DNA sensor using a silicon nanowire array. , 2012, Journal of biotechnology.

[2]  C. Yoon,et al.  Electrochemical albumin sensing based on silicon nanowires modified by gold nanoparticles , 2011 .

[3]  Jun‐Jie Zhu,et al.  Aptamer-quantum dots conjugates-based ultrasensitive competitive electrochemical cytosensor for the detection of tumor cell. , 2011, Talanta.

[4]  Kun-Lin Yang,et al.  Detection of DNA targets hybridized to solid surfaces using optical images of liquid crystals. , 2011, ACS applied materials & interfaces.

[5]  Li Zhang,et al.  Silicon nanowire biosensor for highly sensitive and rapid detection of Dengue virus , 2010 .

[6]  Zhengbo Chen,et al.  Signal amplification architecture for electrochemical aptasensor based on network-like thiocyanuric acid/gold nanoparticle/ssDNA. , 2012, Biosensors & bioelectronics.

[7]  Ajay Agarwal,et al.  Label-free direct detection of MiRNAs with silicon nanowire biosensors. , 2009, Biosensors & bioelectronics.

[8]  Wei Cheng,et al.  A sensitive electrochemical DNA biosensor for specific detection of Enterobacteriaceae bacteria by Exonuclease III-assisted signal amplification. , 2013, Biosensors & bioelectronics.

[9]  Chunhai Fan,et al.  Silicon nanostructures for bioapplications , 2010 .

[10]  Mengsu Yang,et al.  Silicon nanowires for high-sensitivity glucose detection , 2006 .

[11]  Guo Hongxia,et al.  Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE. , 2007, Bioelectrochemistry.

[12]  Allen J. Bard,et al.  Electrochemical Methods: Fundamentals and Applications , 1980 .

[13]  Charles M. Lieber,et al.  Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors , 2004 .

[14]  Tingting Xu,et al.  Silicon nanowires-based highly-efficient SERS-active platform for ultrasensitive DNA detection , 2011 .

[15]  Giovanna Marrazza,et al.  Electrochemical and piezoelectric DNA biosensors for hybridisation detection. , 2008, Analytica chimica acta.

[16]  Tit Meng Lim,et al.  Detection of Saccharomyces cerevisiae immobilized on self-assembled monolayer (SAM) of alkanethiolate using electrochemical impedance spectroscopy , 2005 .

[17]  Chunhai Fan,et al.  Silicon nanowire-based molecular beacons for high-sensitivity and sequence-specific DNA multiplexed analysis. , 2012, ACS nano.

[18]  Fei Gao,et al.  Application of graphene-pyrenebutyric acid nanocomposite as probe oligonucleotide immobilization platform in a DNA biosensor. , 2013, Materials science & engineering. C, Materials for biological applications.

[19]  C. Soeller,et al.  Quantum dots and nanostructured conducting polymers for biosensing applications , 2009 .

[20]  Loïc J. Blum,et al.  DNA Biosensors and Microarrays , 2008 .

[21]  Maotian Xu,et al.  Direct electrochemical sensor for label-free DNA detection based on zero current potentiometry. , 2013, Biosensors & bioelectronics.

[22]  M. F. Mousavi,et al.  A new DNA-nanobiosensor based on G-quadruplex immobilized on carbon nanotubes modified glassy carbon electrode , 2012 .

[23]  S. Girousi,et al.  Detection of short oligonucleotide sequences using an electrochemical DNA hybridization biosensor , 2009, 2009 2nd International Symposium on Applied Sciences in Biomedical and Communication Technologies.

[24]  Jian Zhang,et al.  Nickel hydroxide modified silicon nanowires electrode for hydrogen peroxide sensor applications , 2012 .

[25]  Guonan Chen,et al.  Electrochemical behavior of o-sec-butylphenol at glassy carbon electrode modified with multiwalled carbon nanotubes and 1-butyl-3-methylimidazolium hexafluorophosphate. , 2012, The Analyst.

[26]  G. Shi,et al.  A Sensitive Nanoporous Gold-Based Electrochemical DNA Biosensor for Escherichia coli Detection , 2011 .

[27]  Hadi Nur,et al.  Gold Nanoparticles Embedded on the Surface of Polyvinyl Alcohol Layer , 2014 .

[28]  C. Castelletti,et al.  Electrochemical DNA biosensor for bovine papillomavirus detection using polymeric film on screen-printed electrode. , 2012, Biosensors & bioelectronics.

[29]  Li Wang,et al.  Electrochemical synthesis of gold nanostructure modified electrode and its development in electrochemical DNA biosensor. , 2011, Biosensors & bioelectronics.

[30]  B. Pritt,et al.  Update: The diagnosis and management of dengue virus infection in North America. , 2012, Diagnostic microbiology and infectious disease.

[31]  Chinnasamy Thiruppathiraja,et al.  Specific detection of Mycobacterium sp. genomic DNA using dual labeled gold nanoparticle based electrochemical biosensor. , 2011, Analytical biochemistry.

[32]  Guo-Jun Zhang,et al.  Self-assembled monolayer-assisted silicon nanowire biosensor for detection of protein-DNA interactions in nuclear extracts from breast cancer cell. , 2011, Biosensors & bioelectronics.

[33]  B. D. Malhotra,et al.  DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease. , 2010, Journal of biotechnology.

[34]  Longhua Tang,et al.  Electrochemical DNA sensor by the assembly of graphene and DNA-conjugated gold nanoparticles with silver enhancement strategy. , 2011, The Analyst.

[35]  E. Alocilja,et al.  A multiplex nanoparticle-based bio-barcoded DNA sensor for the simultaneous detection of multiple pathogens. , 2010, Biosensors & bioelectronics.

[36]  H. Ju,et al.  Sub-femtomolar electrochemical detection of DNA using surface circular strand-replacement polymerization and gold nanoparticle catalyzed silver deposition for signal amplification. , 2013, Biosensors & bioelectronics.

[37]  S. Yip,et al.  One-step rapid reverse transcription-PCR assay for detecting and typing dengue viruses with GC tail and induced fluorescence resonance energy transfer techniques for melting temperature and color multiplexing. , 2007, Clinical chemistry.

[38]  Wei Zhang,et al.  Electrochemical determination of BCR/ABL fusion gene based on in situ synthesized gold nanoparticles and cerium dioxide nanoparticles. , 2013, Colloids and surfaces. B, Biointerfaces.

[39]  J. Chen,et al.  Comparing the performances of electrochemical sensors using p-aminophenol redox cycling by different reductants on gold electrodes modified with self-assembled monolayers , 2013 .

[40]  Mehmet Mutlu,et al.  QCM-based DNA biosensor for detection of genetically modified organisms (GMOs) , 2009 .

[41]  Joseph Wang,et al.  An electrochemical RNA hybridization assay for detection of the fecal indicator bacterium Escherichia coli. , 2005, Marine pollution bulletin.

[42]  N. Yusof,et al.  The development of silicon nanowire as sensing material and its applications , 2013 .

[43]  Kagan Kerman,et al.  Recent trends in electrochemical DNA biosensor technology , 2004 .

[44]  N Balasubramanian,et al.  Highly sensitive measurements of PNA-DNA hybridization using oxide-etched silicon nanowire biosensors. , 2008, Biosensors & bioelectronics.

[45]  Mingwang Shao,et al.  Gas sensing of tellurium-modified silicon nanowires to ammonia and propylamine , 2013 .

[46]  K. Shepard,et al.  Label-free single-molecule detection of DNA-hybridization kinetics with a carbon nanotube field-effect transistor. , 2011, Nature nanotechnology.

[47]  Wei Sun,et al.  Electrochemical DNA Biosensor Based on Partially Reduced Graphene Oxide Modified Carbon Ionic Liquid Electrode for the Detection of Transgenic Soybean A2704‐12 Gene Sequence , 2013 .

[48]  T. S. Ramulu,et al.  Nanowires array modified electrode for enhanced electrochemical detection of nucleic acid. , 2013, Biosensors & bioelectronics.

[49]  A. De,et al.  Highly sensitive electrochemical biosensor for glucose, DNA and protein using gold-polyaniline nanocomposites as a common matrix , 2014 .

[50]  N. Yusof,et al.  Bismuth Oxide Nanoparticles/Chitosan/Modified Electrode as Biosensor for DNA Hybridization , 2011, International Journal of Electrochemical Science.

[51]  S. Yao,et al.  Highly sensitive and selective dopamine biosensor based on a phenylethynyl ferrocene/graphene nanocomposite modified electrode. , 2012, The Analyst.

[52]  Adisorn Tuantranont,et al.  DNA hybridization enhancement using piezoelectric microagitation through a liquid coupling medium. , 2011, Lab on a chip.

[53]  A. Erdem,et al.  Electrochemical Monitoring of DNA Hybridization by Multiwalled Carbon Nanotube Based Screen Printed Electrodes , 2008 .

[54]  E. Jacques,et al.  Step-gate polysilicon nanowires field effect transistor compatible with CMOS technology for label-free DNA biosensor. , 2013, Biosensors & bioelectronics.

[55]  R. Stanley Williams,et al.  Silicon nanowires for sequence-specific DNA sensing: device fabrication and simulation , 2005 .

[56]  Jeroen Lammertyn,et al.  Real-time monitoring of DNA hybridization and melting processes using a fiber optic sensor , 2012, Nanotechnology.

[57]  H. Ju,et al.  A label-free electrochemical DNA biosensor based on a Zr(IV)-coordinated DNA duplex immobilised on a carbon nanofibre|chitosan layer , 2012, Analytical and Bioanalytical Chemistry.

[58]  Jian-hui Jiang,et al.  Ultrasensitive electrochemical DNA detection based on dual amplification of circular strand-displacement polymerase reaction and hybridization chain reaction. , 2013, Biosensors & bioelectronics.

[59]  Varun Rai,et al.  Ultrasensitive cDNA Detection of Dengue Virus RNA Using Electrochemical Nanoporous Membrane-Based Biosensor , 2012, PloS one.

[60]  Ling-Fan Zhang,et al.  Electrochemical detection of DNA hybridization by using a zirconia modified renewable carbon paste electrode. , 2009, Bioelectrochemistry.

[61]  A. Srivastava,et al.  Chitosan encapsulated quantum dots platform for leukemia detection. , 2012, Biosensors & bioelectronics.

[62]  A. Erdem,et al.  Electrochemical sensing of label free DNA hybridization related to breast cancer 1 gene at disposable sensor platforms modified with single walled carbon nanotubes , 2012 .

[63]  Wei Sun,et al.  Development of a sensitive electrochemical DNA sensor by 4-aminothiophenol self-assembled on electrodeposited nanogold electrode coupled with Au nanoparticles labeled reporter ssDNA , 2012 .

[64]  Ezat Hamidi-Asl,et al.  Preparation of Ag/NaA zeolite modified carbon paste electrode as a DNA biosensor , 2013 .

[65]  A. Salleh,et al.  Electrochemical DNA biosensor for the detection of specific gene related to Trichoderma harzianum species. , 2010, Bioelectrochemistry.

[66]  Govind,et al.  DNA hybridization on silicon nanowires , 2010 .

[67]  M. A. Mehrgardi,et al.  Nanoporous gold electrode as a platform for the construction of an electrochemical DNA hybridization biosensor. , 2012, Biosensors & bioelectronics.

[68]  Uda Hashim,et al.  The utilization of SiNWs/AuNPs-modified indium tin oxide (ITO) in fabrication of electrochemical DNA sensor. , 2014, Materials science & engineering. C, Materials for biological applications.

[69]  M. Sales,et al.  Gold electrode modified by self-assembled monolayers of thiols to determine DNA sequences hybridization , 2010 .

[70]  T. Łuczak.  Gold Electrodes Modified with Self-Assembled Layers Made of Sulphur Compounds and Gold Nanoparticles Used for Selective Electrocatalytic Oxidation of Catecholamine in the Presence of Interfering Ascorbic and Uric Acids , 2011 .

[71]  Wei Sun,et al.  Electrochemical Behaviors of Methylene Blue on DNA Modified Electrode and Its Application to the Detection of PCR Product from NOS Sequence , 2008, Sensors.

[72]  Farrokh Karimi,et al.  Construction, electrochemically biosensing and discrimination of recombinant plasmid (pEThIL-2) on the basis of interleukine-2 DNA insert. , 2008, Biosensors & bioelectronics.

[73]  M. Islam,et al.  Highly sensitive electrolyte-insulator-semiconductor pH sensors enabled by silicon nanowires with Al2O3/SiO2 sensing membrane , 2012 .