Fluorescent nanodiamonds for ultrasensitive detection

Fluorescent nanodiamonds (NDs) are new and emerging nanomaterials that have potential to be used as fluorescence imaging agents and also as a highly versatile platform for the controlled functionalization and delivery of a wide spectrum of therapeutic agents. We will utilize two experimental methods, TIRF, a relatively simple method based on total internal reflection fluorescence and SPRF, fluorescence enhanced by resonance coupling with surface plasmons. We estimate that the SPRF method will be 100 times sensitive than currently available similar detectors based on detectors. The ultimate goal of this research is to develop microarray platforms that could be used for sensitive, fast and inexpensive gene sequencing and protein detection.

[1]  I. Gryczynski,et al.  Ratiometric FRET-based detection of DNA and micro-RNA on the surface using TIRF detection , 2010 .

[2]  Jui‐I Chao,et al.  Covalent linkage of nanodiamond-paclitaxel for drug delivery and cancer therapy , 2010, Nanotechnology.

[3]  E. Perevedentseva,et al.  Effect of surface adsorbed proteins on the photoluminescence of nanodiamond , 2011 .

[4]  R. Kalish,et al.  Nitrogen doping of diamond by ion implantation , 1997 .

[5]  Yury Gogotsi,et al.  The properties and applications of nanodiamonds. , 2011, Nature nanotechnology.

[6]  Brown,et al.  Electronic structure of the N-V center in diamond: Experiments. , 1996, Physical review. B, Condensed matter.

[7]  Houjin Huang,et al.  Protein-mediated assembly of nanodiamond hydrogels into a biocompatible and biofunctional multilayer nanofilm. , 2008, ACS nano.

[8]  S. Tugarinov,et al.  Transformation of GR1 defects in annealed natural type IIa diamonds , 2000 .

[9]  Erik Pierstorff,et al.  Active nanodiamond hydrogels for chemotherapeutic delivery. , 2007, Nano letters.

[10]  M. F. Hamer,et al.  Optical studies of the 1.945 eV vibronic band in diamond , 1976, Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences.

[11]  Huan-Cheng Chang,et al.  Mass production and dynamic imaging of fluorescent nanodiamonds. , 2008, Nature nanotechnology.

[12]  J. Twamley,et al.  Observation and control of blinking nitrogen-vacancy centres in discrete nanodiamonds. , 2010, Nature nanotechnology.

[13]  V. V. Danilenko,et al.  On the history of the discovery of nanodiamond synthesis , 2004 .

[14]  Adam Gali,et al.  Theory of the neutral nitrogen-vacancy center in diamond and its application to the realization of a qubit , 2009 .

[15]  H-C Chang,et al.  Functionalized fluorescent nanodiamonds for biomedical applications. , 2009, Nanomedicine.

[16]  J. Rarity,et al.  Nanofabricated solid immersion lenses registered to single emitters in diamond , 2010, 1012.1135.