Preparation and characterization of water-soluble chitosan nanoparticles as protein delivery system

The objective of this study was to investigate the potential of water soluble chitosan as a carrier in the preparation of protein-loaded nanoparticles. Nanoparticles were prepared by ionotropic gelation of water-soluble chitosan (WSC) with sodium tripolyphosphate (TPP). Bovine serum albumin (BSA) was applied as a model drug. The size and morphology of the nanoparticles were investigated as a function of the preparation conditions. The particles were spherical in shape and had a smooth surface. The size range of the nanoparticles was between 100 and 400 nm. Result of the in vitro studies showed that the WSC nanoparticles enhance and prolong the intestinal absorption of BSA. These results also indicated that WSC nanoparticles were a potential protein delivery system.

[1]  Zhirong Zhang,et al.  Evaluation and modification of N-trimethyl chitosan chloride nanoparticles as protein carriers. , 2007, International journal of pharmaceutics.

[2]  Cui Tang,et al.  Chitosan graft copolymer nanoparticles for oral protein drug delivery: preparation and characterization. , 2006, Biomacromolecules.

[3]  C. Remuñán-López,et al.  Microencapsulated chitosan nanoparticles for lung protein delivery. , 2005, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[4]  Daniel E. Otzen,et al.  Protein drug stability: a formulation challenge , 2005, Nature Reviews Drug Discovery.

[5]  Kinam Park,et al.  Bioadhesive interaction and hypoglycemic effect of insulin-loaded lectin-microparticle conjugates in oral insulin delivery system. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[6]  K. Janes,et al.  Depolymerized chitosan nanoparticles for protein delivery: Preparation and characterization , 2003 .

[7]  Eugene Khor,et al.  Implantable applications of chitin and chitosan. , 2003, Biomaterials.

[8]  K. Zhu,et al.  Controlled drug release properties of ionically cross-linked chitosan beads: the influence of anion structure. , 2002, International journal of pharmaceutics.

[9]  H. Junginger,et al.  Oral drug absorption enhancement by chitosan and its derivatives. , 2001, Advanced drug delivery reviews.

[10]  R. Vandenberg,et al.  Glycine transport inhibitors as potential antipsychotic drugs , 2001, Expert opinion on therapeutic targets.

[11]  K. Janes,et al.  Polysaccharide colloidal particles as delivery systems for macromolecules. , 2001, Advanced drug delivery reviews.

[12]  Y. Kawashima,et al.  Mucoadhesive nanoparticulate systems for peptide drug delivery. , 2001, Advanced drug delivery reviews.

[13]  M. Akashi,et al.  Design of nanoparticles composed of graft copolymers for oral peptide delivery. , 2001, Advanced drug delivery reviews.

[14]  A. Lamprecht,et al.  Influences of process parameters on nanoparticle preparation performed by a double emulsion pressure homogenization technique. , 2000, International journal of pharmaceutics.

[15]  E. Mathiowitz,et al.  Nanosphere based oral insulin delivery. , 2000, Journal of controlled release : official journal of the Controlled Release Society.

[16]  S. Hudson,et al.  Improved mechanical properties of chitosan fibers , 1999 .

[17]  F. Forni,et al.  Dynamic dialysis for the drug release evaluation from doxorubicin-gelatin nanoparticle conjugates. , 1999, International journal of pharmaceutics.

[18]  J. Irache,et al.  Specific and non-specific bioadhesive particulate systems for oral delivery to the gastrointestinal tract. , 1998, Advanced drug delivery reviews.

[19]  M. Alonso,et al.  Novel hydrophilic chitosan‐polyethylene oxide nanoparticles as protein carriers , 1997 .

[20]  L. Jorgensen,et al.  Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles. , 2007, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[21]  Y. Matsumoto,et al.  Enzymatic and hyperglycemia stability of chemically modified insulins with hydrophobic acyl groups. , 2004, Bioorganic & medicinal chemistry letters.

[22]  W. Paul,et al.  Chitosan, a drug carrier for the 21st century : a review , 2000 .