Spherule coccidioidin in delayed dermal sensitivity reactions of experimental animals.

A new coccidioidin, derived from the spherule phase of Coccidioides immitis cultured in vitro, was examined for its capacity to elicit delayed dermal hypersensitivity reactions in mice and guinea pigs. Mycelial coccidioidin, widely used in diagnostic procedures, was studied in parallel. The animals were sensitized either by infection with arthrospores or by vaccination with formalin-killed spherules or mycelium; sensitivity reactions were determined 3–5 weeks later or, in 1 experiment, afte 18 months. The capacities of the preparations to detect reactions augmented anamnestically by a booster dose of vaccine given 18 months after original sensitization were compared, as well as reactions induced with a strain of C. immitis not used in the preparation of either reagent.In each comparison above, the spherule coccidioidin was judged to be more sensitive than the mycelial in that a lower dose (1) elicited stronger reactions, (2) showed higher ratios of sensitized animals to be reactive, and (3) induced reacti...

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