The Role of Drosophila Mushroom Body Signaling in Olfactory Memory

The mushroom bodies of the Drosophila brain are important for olfactory learning and memory. To investigate the requirement for mushroom body signaling during the different phases of memory processing, we transiently inactivated neurotransmission through this region of the brain by expressing a temperature-sensitive allele of the shibire dynamin guanosine triphosphatase, which is required for synaptic transmission. Inactivation of mushroom body signaling through α/β neurons during different phases of memory processing revealed a requirement for mushroom body signaling during memory retrieval, but not during acquisition or consolidation.

[1]  K. Ikeda,et al.  Disappearance and reformation of synaptic vesicle membrane upon transmitter release observed under reversible blockage of membrane retrieval , 1989, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[2]  M Heisenberg,et al.  Localization of a short-term memory in Drosophila. , 2000, Science.

[3]  R. Davis,et al.  Tripartite mushroom body architecture revealed by antigenic markers. , 1998, Learning & memory.

[4]  C. D. Beck,et al.  Learning Performance of Normal and MutantDrosophila after Repeated Conditioning Trials with Discrete Stimuli , 2000, The Journal of Neuroscience.

[5]  M Heisenberg,et al.  Associative odor learning in Drosophila abolished by chemical ablation of mushroom bodies. , 1994, Science.

[6]  W. Quinn,et al.  Radish, a Drosophila mutant deficient in consolidated memory. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Ronald L. Davis,et al.  Drosophila fasciclinII Is Required for the Formation of Odor Memories and for Normal Sensitivity to Alcohol , 2001, Cell.

[8]  L. Luo,et al.  Development of the Drosophila mushroom bodies: sequential generation of three distinct types of neurons from a neuroblast. , 1999, Development.

[9]  W. Quinn,et al.  The amnesiac Gene Product Is Expressed in Two Neurons in the Drosophila Brain that Are Critical for Memory , 2000, Cell.

[10]  Tim Tully,et al.  Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory , 2001, Nature.

[11]  Tim Tully,et al.  Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies , 1996, Science.

[12]  M. Heisenberg.  What do the mushroom bodies do for the insect brain? an introduction. , 1998, Learning & memory.

[13]  N. Perrimon,et al.  Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. , 1993, Development.

[14]  A Borst,et al.  Drosophila mushroom body mutants are deficient in olfactory learning. , 1985, Journal of neurogenetics.

[15]  Richard B. Vallee,et al.  Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis , 1991, Nature.

[16]  F. Kawasaki,et al.  Fast synaptic fatigue in shibire mutants reveals a rapid requirement for dynamin in synaptic vesicle membrane trafficking , 2000, Nature Neuroscience.

[17]  M. Ramaswami,et al.  Probable mechanisms underlying interallelic complementation and temperature-sensitivity of mutations at the shibire locus of Drosophila melanogaster. , 1998, Genetics.

[18]  T. Kitamoto.  Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons. , 2001, Journal of neurobiology.

[19]  Ronald L. Davis.  Mushroom bodies and drosophila learning , 1993, Neuron.

[20]  Alexander M. van der Bliek,et al.  Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic , 1991, Nature.