Rotational multiphoton endoscopy with a 1 μm fiber laser system

We present multiphoton microendoscopy with a rotational probe and a 1 μm fiber-based femtosecond laser. The rotational probe is based on a double-clad photonic crystal fiber, a gradient index lens, a microprism, and a rotational microelectronicmechanical system (MEMS) motor. The MEMS motor has a diameter of 2.2 mm and can provide 360° full-view rotation. The fiber laser provides ultrashort pulses with a central wavelength at 1.034 μm and a repetition rate of 50 MHz. Second-harmonic-generation images of rat-tail tendon and fish scale are demonstrated with the rotational probe-based multiphoton system.

[1]  Jianping Su,et al.  Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy. , 2008, Optics letters.

[2]  F. Helmchen,et al.  Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo. , 2008, Optics express.

[3]  Jun Zhang,et al.  In vivo three-dimensional microelectromechanical endoscopic swept source optical coherence tomography. , 2007, Optics express.

[4]  Xingde Li,et al.  Fiber-optic scanning two-photon fluorescence endoscope. , 2006, Optics letters.

[5]  W. Denk,et al.  Deep tissue two-photon microscopy , 2005, Nature Methods.

[6]  E. Cocker,et al.  Fiber-optic fluorescence imaging , 2005, Nature Methods.

[7]  Ling Fu,et al.  Nonlinear optical microscopy based on double-clad photonic crystal fibers. , 2005, Optics express.

[8]  F. Helmchen,et al.  Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective. , 2004, Optics letters.

[9]  P. Russell,et al.  Enhanced two-photon biosensing with double-clad photonic crystal fibers. , 2003, Optics letters.

[10]  A. Mehta,et al.  Multiphoton endoscopy: optical design and application to in vivo imaging of mammalian hippocampal neurons , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..

[11]  F. Wise,et al.  Generation of 2-nJ pulses from a femtosecond ytterbium fiber laser. , 2003, Optics letters.

[12]  P. So,et al.  Handbook of Biomedical Nonlinear Optical Microscopy , 2009 .

[13]  Tommaso Baldacchini,et al.  Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media. , 2009, Journal of biomedical optics.