DROP-ON-DEMAND INKJET BIOPRINTING: A PRIMER ∗

Engineering complex biological structures for regenerative medicine, in vitro tissue analysis, and pharmaceutical testing require new fabrication techniques that can place specific cells in specific target locations. Conventional cell seeding methods cannot achieve this level of spatial resolution. Biofabrication is a rapidly advancing field that uses a variety of delivery mechanisms to achieve the spatial resolution necessary to place cells, biomaterials, and bioactive macromolecules in specific target locations. One new technique within this field is bioprinting, which uses drop-on-demand delivery mechanisms to fabricate biological structures. This review focuses on drop-on-demand inkjet bioprinting and provides a primer for researchers seeking to enter the field.

[1]  Vladimir Mironov,et al.  Organ printing: tissue spheroids as building blocks. , 2009, Biomaterials.

[2]  Tao Xu,et al.  Inkjet-mediated gene transfection into living cells combined with targeted delivery. , 2009, Tissue engineering. Part A.

[3]  Tao Xu,et al.  High-Throughput Production of Single-Cell Microparticles Using an Inkjet Printing Technology , 2008 .

[4]  Todd D. Giorgio,et al.  Mitosis enhances transgene expression of plasmid delivered by cationic liposomes , 1999, Proceedings of the First Joint BMES/EMBS Conference. 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Annual Fall Meeting of the Biomedical Engineering Society (Cat. N.

[5]  O. Hermanson,et al.  Inkjet printing of macromolecules on hydrogels to steer neural stem cell differentiation. , 2007, Biomaterials.

[6]  A. Miller,et al.  Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection , 1990, Molecular and cellular biology.

[7]  P. Vermette,et al.  Scaffold vascularization: a challenge for three-dimensional tissue engineering. , 2010, Current medicinal chemistry.

[8]  Anthony Atala,et al.  Tissue engineering and regenerative medicine: concepts for clinical application. , 2004, Rejuvenation research.

[9]  T. Boland,et al.  Cell damage evaluation of thermal inkjet printed Chinese hamster ovary cells , 2010, Biotechnology and bioengineering.

[10]  Vladimir Mironov,et al.  Organ printing: computer-aided jet-based 3D tissue engineering. , 2003, Trends in biotechnology.

[11]  Tao Xu,et al.  Construction of high‐density bacterial colony arrays and patterns by the ink‐jet method , 2004, Biotechnology and bioengineering.

[12]  V. Mironov,et al.  Engineering biological structures of prescribed shape using self-assembling multicellular systems. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Matthew E. Pepper,et al.  EDTA enhances high‐throughput two‐dimensional bioprinting by inhibiting salt scaling and cell aggregation at the nozzle surface , 2009, Journal of tissue engineering and regenerative medicine.

[14]  S. Yoo,et al.  On‐demand three‐dimensional freeform fabrication of multi‐layered hydrogel scaffold with fluidic channels , 2010, Biotechnology and bioengineering.

[15]  和田 八三久 Materials science. , 1973, Science.

[16]  Robert J. Klebe,et al.  Cytoscription: Computer controlled micropositioning of cell adhesion proteins and cells , 1994 .

[17]  T. Boland,et al.  Human microvasculature fabrication using thermal inkjet printing technology. , 2009, Biomaterials.

[18]  John P Fisher,et al.  Tubular perfusion system for the long-term dynamic culture of human mesenchymal stem cells. , 2011, Tissue engineering. Part C, Methods.

[19]  Bradley R. Ringeisen,et al.  Laser Printing of Single Cells: Statistical Analysis, Cell Viability, and Stress , 2005, Annals of Biomedical Engineering.

[20]  Walker Inman,et al.  Liver tissue engineering in the evaluation of drug safety , 2009, Expert opinion on drug metabolism & toxicology.

[21]  J A Barron,et al.  Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns , 2004, Biomedical microdevices.

[22]  R. Pont-Lezica,et al.  Localizing proteins by tissue printing. , 2009, Methods in molecular biology.

[23]  R. Klebe,et al.  Cytoscribing: a method for micropositioning cells and the construction of two- and three-dimensional synthetic tissues. , 1988, Experimental cell research.

[24]  T. Boland,et al.  Inkjet printing of viable mammalian cells. , 2005, Biomaterials.

[25]  Michele Marcolongo,et al.  Characterization of cell viability during bioprinting processes. , 2009, Biotechnology journal.

[26]  Eric D. Miller,et al.  Inkjet-based biopatterning of bone morphogenetic protein-2 to spatially control calvarial bone formation. , 2010, Tissue engineering. Part A.

[27]  L. Niklason,et al.  Scaffold-free vascular tissue engineering using bioprinting. , 2009, Biomaterials.

[28]  F. Gage,et al.  In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector , 1996, Science.

[29]  Wei Sun,et al.  Effects of Dispensing Pressure and Nozzle Diameter on Cell Survival from Solid Freeform Fabrication–Based Direct Cell Writing , 2008 .

[30]  Markus Uhrberg,et al.  Rapid and highly efficient gene transfer into natural killer cells by nucleofection. , 2003, Journal of immunological methods.

[31]  F. Guillemot,et al.  High-throughput laser printing of cells and biomaterials for tissue engineering. , 2010, Acta biomaterialia.

[32]  Sangeeta N Bhatia,et al.  Engineering tissues for in vitro applications. , 2006, Current opinion in biotechnology.

[33]  W Cris Wilson,et al.  Cell and organ printing 1: protein and cell printers. , 2003, The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology.

[34]  Adrian Neagu,et al.  Organ printing: fiction or science. , 2004, Biorheology.

[35]  Tao Xu,et al.  Viability and electrophysiology of neural cell structures generated by the inkjet printing method. , 2006, Biomaterials.

[36]  I. Morita,et al.  Biocompatible inkjet printing technique for designed seeding of individual living cells. , 2005, Tissue engineering.

[37]  Anthony Atala,et al.  Engineering organs. , 2009, Current opinion in biotechnology.

[38]  Fyodor D Urnov,et al.  Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases , 2007, Proceedings of the National Academy of Sciences.

[39]  P. Calvert Printing Cells , 2007, Science.

[40]  Stuart K Williams,et al.  Three-dimensional bioassembly tool for generating viable tissue-engineered constructs. , 2004, Tissue engineering.

[41]  Jeffrey C. Miller,et al.  Highly efficient endogenous human gene correction using designed zinc-finger nucleases , 2005, Nature.

[42]  Kenneth M. Yamada,et al.  Modeling Tissue Morphogenesis and Cancer in 3D , 2007, Cell.

[43]  Fabien Guillemot,et al.  Laser-assisted cell printing: principle, physical parameters versus cell fate and perspectives in tissue engineering. , 2010, Nanomedicine.

[44]  Xiaofeng Cui,et al.  Application of inkjet printing to tissue engineering , 2006, Biotechnology journal.

[45]  Jong-Hwan Lee,et al.  Three-dimensional bioprinting of rat embryonic neural cells , 2009, Neuroreport.

[46]  T. Boland,et al.  Inkjet printing for high-throughput cell patterning. , 2004, Biomaterials.

[47]  W. Hennink,et al.  Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. , 2007, Tissue engineering.

[48]  Eric D. Miller,et al.  Microenvironments Engineered by Inkjet Bioprinting Spatially Direct Adult Stem Cells Toward Muscle‐ and Bone‐Like Subpopulations , 2008, Stem cells.

[49]  A. Khademhosseini,et al.  Layer by layer three-dimensional tissue epitaxy by cell-laden hydrogel droplets. , 2010, Tissue engineering. Part C, Methods.

[50]  Nina Desai,et al.  Three-dimensional in vitro follicle growth: overview of culture models, biomaterials, design parameters and future directions , 2010, Reproductive biology and endocrinology : RB&E.

[51]  W. Dhert,et al.  Three-dimensional fiber deposition of cell-laden, viable, patterned constructs for bone tissue printing. , 2008, Tissue engineering. Part A.

[52]  Matthew E. Pepper,et al.  Building off-the-shelf tissue-engineered composites , 2010, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[53]  Wei Sun,et al.  Microprinting of liver micro-organ for drug metabolism study. , 2011, Methods in molecular biology.

[54]  Anthony Atala,et al.  Engineering Complex Tissues , 2012, Science Translational Medicine.

[55]  Elikplimi K. Asem,et al.  Three-Dimensional Cell Culture to Model Epithelia in the Female Reproductive System , 2007, Reproductive Sciences.