Cell Elongation and Revolving Movement in Phaseolus vulgaris L. Twining Shoots

In Phaseolus vulgaris L., the shoot displays a revolving movement that occurs rhythmically in a highly regular manner. Previous data led to think that revolving movement is driven by turgor and volume changes in the epidermal cells of the bending zone. To document this hypothesis, the time course of in situ cell length variations in the bending zone was measured during the movement of the shoot and related to the phase of the revolving movement. Each ten minutes, a photograph of cells was taken and the revolving movement was simultaneously recorded using time-lapse microphotography and video-monitoring. In the moving part of the shoot, epidermal cells displayed partly reversible length variations during their growth. Data were processed by Fourier analysis to determine whether or not a periodicity exists. Rhythm in cell length variations was evidenced only when initial cell lengths were ranged between 60 and 120 fan. In this case, the period corresponds to that of the revolving movement.

[1]  D J Cosgrove,et al.  Wall relaxation and the driving forces for cell expansive growth. , 1987, Plant physiology.

[2]  Yasushi Hashimoto,et al.  Observation of Stomatal Movements of Intact Plants Using an Image Instrumentation System with a Light Microscope , 1983 .

[3]  S. McQueen-Mason,et al.  Two endogenous proteins that induce cell wall extension in plants. , 1992, The Plant cell.

[4]  P. Jolliffe,et al.  High-resolution studies of growth oscillations during stem elongation , 1986 .

[5]  R. Cleland.  Cell Wall Extension , 1971 .

[6]  J Mercier,et al.  Rhythmic circumnutation movement of the shoots in Phaseolus vulgaris L. , 1984, Chronobiology international.

[7]  J. Derksen.  Pollen Tubes: a Model System for Plant Cell Growth* , 1996 .

[8]  J. Boyer,et al.  Complete turgor maintenance at low water potentials in the elongating region of maize leaves. , 1982, Plant physiology.

[9]  P. Bannister,et al.  Pressure and Solute Potentials in Stomatal Cells of Tradescantia virginiana , 1979 .

[10]  Stephen C. Fry,et al.  Turgor and Cell Expansion: Beyond the Lockhart Equation , 1992 .

[11]  P. M. Badot,et al.  Circumnutation in Phaseolus vulgaris. II. Potassium content in the free-moving part of the shoot. , 1990 .

[12]  P. Badot,et al.  Circumnutation in Phaseolus vulgaris. I. Growth, osmotic potential and cell ultrastructure in the free-moving part of the shoot , 1988 .

[13]  P. B. Green,et al.  High‐resolution measurement of growth during first positive phototropism in maize , 1985 .

[14]  D. Cosgrove.  Relaxation in a high-stress environment: the molecular bases of extensible cell walls and cell enlargement. , 1997, The Plant cell.

[15]  S. McQueen-Mason,et al.  Expansin Mode of Action on Cell Walls (Analysis of Wall Hydrolysis, Stress Relaxation, and Binding) , 1995, Plant physiology.

[16]  C. Darwin.  The Movements and Habits of Climbing Plants , 1875, Nature.

[17]  U. Kutschera,et al.  Sucrose metabolism and cell elongation in developing sunflower hypocotyls , 1995 .